Rabbit anti-Mouse CD56/NCAM Monoclonal Antibody for FC - P13595

Core Product Specifications and Parameters

Biological Background: NCAM1 Function and Localization

• NCAM1 (Neural Cell Adhesion Molecule 1), also known as CD56, is a cell adhesion molecule that mediates neuron-neuron adhesion, essential for neurite fasciculation and outgrowth.
• Mouse NCAM1 exists as multiple isoforms generated by alternative splicing, with molecular weights of 67, 80, 93, and 119 kDa.
• It belongs to the immunoglobulin superfamily and contains immunoglobulin-like domains and fibronectin type III repeats.
• The protein is localized to the cell membrane and can exist as transmembrane or GPI-anchored forms.
• NCAM1 is heavily glycosylated and subject to phosphorylation, which modulate its adhesive properties.
• It plays critical roles in neural development, including cell migration, synapse formation, and plasticity.

Experimental Guidance and Technical Tips

• As a fluorochrome-conjugated antibody, it is recommended to titrate the antibody to determine the optimal concentration for flow cytometry to achieve the best signal-to-noise ratio.
• Include appropriate isotype controls and fluorescence-minus-one (FMO) controls to ensure specific staining.
• Validate the antibody in the relevant mouse tissue or cell line, considering that NCAM1 expression may vary among cell types and developmental stages.
• Due to the presence of multiple isoforms, ensure that the detection system can resolve populations if differential expression is expected.
• Protect the antibody from light and store at 2–8°C, avoiding freeze-thaw cycles to maintain fluorophore stability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio stands as a dedicated one-stop sourcing partner, providing diagnostic manufacturers, laboratories, and research institutes with comprehensive access to IVD raw materials and technical consultation—from concept through clinical application. This anti-mouse CD56/NCAM antibody supports neuroscience and cell adhesion research, and CamelBio can assist in integrating it into your assay development workflow. Beyond this product, CamelBio offers validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to streamline your IVD raw material procurement.