Rabbit anti-Mouse CD50/ICAM-3 Monoclonal Antibody for FC - Q60625

Core Product Specifications and Parameters

Biological Background: Intercellular Adhesion Molecule 5 (ICAM-5) Function and Localization

• ICAM-5, also known as telencephalin and CD50 in mouse, is a type I transmembrane glycoprotein belonging to the immunoglobulin superfamily.
• It serves as a ligand for the integrin LFA-1 (ITGAL:ITGB2), facilitating neuron–leukocyte adhesion (By similarity).
• Homophilic ICAM-5 interactions promote dendrite outgrowth and arborization in hippocampal neurons. Related references: PMID:22187327
• The protein contains multiple immunoglobulin-like domains with conserved disulfide bonds and predicted N-glycosylation and phosphorylation sites.
• Subcellular localization includes the cell membrane, with enrichment in dendritic spine membranes, postsynaptic densities, and the uropodium of migrating cells.
• Tissue expression is restricted to telencephalic regions of the brain, including the cerebral cortex and hippocampus.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal extracellular region (aa 32-227), suggesting suitability for detecting surface ICAM-5 on live cells in flow cytometry.
• Direct conjugation to allows one-step fluorescent detection; consider optimizing the antibody dilution for your specific cell type and staining protocol.
• As the antibody is raised against mouse ICAM-5, cross-reactivity with other species is not predicted; validate in the relevant sample system if needed.
• The calculated molecular weight of unglycosylated ICAM-5 is approximately 97 kDa; observe appropriate gel conditions if performing confirmatory western blotting (note: validated application is FC only).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-mouse CD50/ICAM-3 monoclonal antibody is part of our expanding portfolio of neural and immune cell markers. Our supply capabilities include validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and customized rare-target sourcing to support your diagnostic and research workflows.