Rabbit anti-Mouse CD5 Monoclonal Antibody for Flow Cytometry - P13379

Core Product Specifications and Parameters

Biological Background: CD5 Function and Localization

• CD5 is a lymphoid-specific receptor expressed on all T-cells and the B1a subset of B-cells, playing a role in TCR/BCR signaling regulation, thymocyte selection, and immune tolerance. Related references: PMID:35720357
• It interacts with B-cell ligands CD5L and CD72, mediating contact-dependent T-cell help for B-cells and maintaining regulatory T/B-cell homeostasis. Related references: PMID:24950378, PMID:9064341, PMID:9723705
• CD5 functions as a negative regulator of TCR signaling during thymocyte development by recruiting signaling proteins such as LCK, CD3ζ, PI3K, and CBL. Related references: PMID:11157848
• CD5 co-engagement with CD3 promotes CBL phosphorylation and VAV1 degradation, attenuating downstream signaling (by similarity).
• In B1a cells, CD5 modulates B-cell activation through TRPC1-mediated Ca²⁺ influx, leading to ERK1/2 activation and IL-10 production (by similarity).
• The protein localizes to the cell membrane as a single-pass type I transmembrane glycoprotein with extracellular scavenger receptor cysteine-rich repeats, a transmembrane helix, and a cytoplasmic tail containing phosphorylation sites.
• CD5 is also known as Ly-1, Ly-A, Ly-12, or Lyt-1 and serves as a pan-T-cell marker and B1a cell marker in mice.

Experimental Guidance and Technical Tips

• The immunogen encompasses amino acids 25-371 of mouse CD5, corresponding to the entire extracellular domain, allowing recognition of native surface CD5 on viable cells without permeabilization.
• For flow cytometry, perform a dilution series (e.g., 1:25 – 1:200) to determine the optimal staining concentration for your cell sample.
• Use appropriate isotype-matched control antibodies and include viability dyes to discriminate live/dead cells and minimize background.
• Protect the antibody from light during staining and storage, as repeated exposure may reduce fluorescence.
• Standard staining protocols recommend incubating 10⁶ cells in 100 µL of staining buffer for 20–30 minutes on ice in the dark, followed by washing.
• This antibody is specific for mouse CD5; cross-reactivity with other species has not been reported; validation in your target tissue is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a trusted one-stop sourcing partner for diagnostic manufacturers and research laboratories, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents for immunology and cell phenotyping workflows. For T-cell and B-cell immunophenotyping, CamelBio provides ready-to-use conjugated antibodies like this anti-CD5 () and supports custom bulk supply, matched antibody pairs, and technical consultations to streamline your IVD assay development from concept to clinic.