Rabbit anti-Mouse CD5 Monoclonal Antibody for Flow Cytometry - P13379

Core Product Specifications and Parameters

Biological Background: CD5 Function and Localization

• CD5 is a lymphoid-specific receptor expressed on all T-cells and a subset of B-cells (B1a cells), also known as Ly-1.

• Functions as a negative regulator of TCR signaling during thymocyte development by interacting with signaling proteins such as LCK, CD3ζ, PI3K, and CBL. Related references: PMID:11157848

• Involved in T-dependent B-cell activation through interaction with CD5L and CD72, contributing to regulatory T and B-cell homeostasis. Related references: PMID:24950378 PMID:9064341 PMID:9723705

• Modulates B-cell biology via ERK1/2 activation in a Ca2+-dependent pathway through TRPC1, leading to IL-10 production.

• Localizes to the cell membrane as a single-pass type I transmembrane protein.

• Contains a signal peptide, three scavenger receptor cysteine-rich (SRCR) repeats in the extracellular domain, and a cytoplasmic tail; it is glycosylated and phosphorylated.

• Regulates thymocyte selection, T-cell effector differentiation, and immune tolerance. Related references: PMID:35720357

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular region (aa 25-371), so the antibody likely recognizes a cell surface epitope, making it well-suited for flow cytometry (FC) without permeabilization.
• For mouse samples, validate expression on T-cells (positive) and B1a B-cells; may also detect low levels on other lymphocyte subsets.
• Use appropriate isotype controls and Fc receptor blocking to minimize non-specific binding.
• Optimize antibody concentration by titration for each cell type.
• Consider co-staining with other T-cell markers (e.g., CD3, CD4, CD8) to characterize T-cell subsets.
• As a monoclonal rabbit antibody, it may offer high specificity and affinity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with streamlined access to high-quality IVD raw materials, including this anti-CD5 monoclonal antibody for mouse immunology studies. Our comprehensive portfolio covers validated antibody pairs, optimized antibodies, bulk ancillary reagents, and custom sourcing of rare targets to support your assay development and manufacturing needs.