Rabbit anti-Mouse CD5 Monoclonal Antibody for FC - P13379

Core Product Specifications and Parameters

Biological Background: CD5 Function and Localization

• CD5 is a lymphoid-specific receptor expressed by all T-cells and a subset of B-cells known as B1a cells.
• It plays a role in regulating TCR and BCR signaling, thymocyte selection, T-cell effector differentiation, and immune tolerance. Related references: PMID:35720357
• CD5 interacts with ligands on B-cells such as CD5L or CD72, contributing to contact-mediated, T-dependent B-cell activation and maintenance of regulatory T and B-cell homeostasis. Related references: PMID:24950378, PMID:9064341, PMID:9723705
• It functions as a negative regulator of TCR signaling during thymocyte development by associating with signaling proteins including LCK, CD3Z chain, PI3K, or CBL. Related references: PMID:11157848
• Mechanistically, co-engagement of CD3 with CD5 enhances phosphorylated CBL recruitment, leading to increased VAV1 phosphorylation and degradation.
• CD5 modulates B-cell biology through ERK1/2 activation in a Ca(2+)-dependent pathway via the non-selective Ca(2+) channel TRPC1, leading to IL-10 production.
• CD5 is located at the cell membrane and possesses key structural features including a disulfide bond, glycosylation, and phosphorylation sites; it acts as a receptor with signal-transducing and transmembrane domains.

Experimental Guidance and Technical Tips

• This antibody is directly conjugated to , enabling one-step staining for flow cytometry without a secondary antibody. Use appropriate laser excitation (e.g., 650 nm) and emission filters (660/20 nm or equivalent).
• Validate the antibody in your experimental system using relevant mouse cell lines or primary cells, such as splenocytes or lymph node lymphocytes, which contain T cells and B1a cells.
• Perform Fc receptor blocking if non-specific binding is observed, particularly when working with myeloid-rich samples.
• Titrate the antibody to determine the optimal concentration for your specific assay conditions, balancing signal-to-noise ratio and minimizing background.
• Consider including appropriate isotype controls and single-color compensation controls for multicolor flow cytometry panels.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with streamlined access to high-quality IVD raw materials, including conjugated antibodies like this CD5 mAb for T-cell and B1a cell studies. Our portfolio covers validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to support your assay development from concept to clinic. Partner with CamelBio for reliable, cost-effective procurement solutions tailored to your workflow needs.