Rabbit anti-Mouse CD48 Monoclonal Antibody for Flow Cytometry - P18181

Core Product Specifications and Parameters

Biological Background: CD48 Function and Localization

• CD48 (SLAMF2) is a GPI-anchored membrane protein of the immunoglobulin superfamily, also known as BCM1, BLAST-1, or sgp-60.
• It engages CD2 on T cells to orchestrate TCR signaling by recruiting LCK and LAT, leading to LCK phosphorylation and T-cell activation (PubMed:9881969). Related references: PMID:9881969
• CD48 binds CD244 (2B4) on NK cells and CD8+ T cells, triggering ITSM phosphorylation and promoting cytolytic granule release for target cell killing (PubMed:21278219). Related references: PMID:21278219
• The protein is predominantly found in the cell membrane, enriched in lipid rafts, and can also exist in a secreted form.
• It contains key structural elements: a signal peptide, Ig-like domains, disulfide bonds, and is glycosylated and GPI-anchored.
• The calculated molecular weight is ~27 kDa (27383 Da from sequence).

Experimental Guidance and Technical Tips

• For flow cytometry applications, titrate the antibody to determine the optimal working concentration for your specific cell type.
• Since the immunogen encompasses the extracellular domain (aa 23-216), this antibody is suitable for detecting cell surface CD48 on live, unfixed cells.
• Cross-reactivity has been confirmed for mouse; always include appropriate isotype and cell controls in your experiments.
• Consider using cell stimulation protocols to upregulate CD48 expression if baseline levels are low in resting lymphocytes.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio simplifies your IVD raw material procurement by offering a comprehensive portfolio of monoclonal antibodies, recombinant proteins, and ancillary reagents for immunology research. Whether you need validated antibody pairs, bulk production of CD48-targeting tools, or rare target sourcing, our supply network can support your development from concept to clinic. Let CamelBio be your trusted partner in accelerating diagnostic assay development.