Rabbit anti-Mouse CD48 Monoclonal Antibody for FC - P18181

Core Product Specifications and Parameters

Biological Background: CD48 Function and Localization

• CD48 is a glycosylphosphatidylinositol (GPI)-anchored cell surface glycoprotein belonging to the signaling lymphocytic activation molecule (SLAM) family, also known as BCM1, BLAST-1, and SLAMF2.
• It contains an N-terminal immunoglobulin domain that mediates interactions with receptors CD2 and CD244 (2B4), regulating immune cell activation and function.
• Through CD2 binding, CD48 facilitates recruitment of the Src kinase LCK and the adaptor LAT to the TCR/CD3 complex, promoting LCK phosphorylation and T-cell signaling. Related references: PMID:21278219 PMID:9881969
• Engagement of CD244 by CD48 induces phosphorylation of immunoreceptor tyrosine-based switch motifs (ITSMs) in CD244, triggering signaling cascades that lead to immunological synapse formation and directed release of perforin and granzymes by T-lymphocytes and NK cells. Related references: PMID:21278219 PMID:9881969
• CD48 is predominantly localized to the cell membrane and membrane rafts, but can also be secreted.
• Its structure features include disulfide bonds, immunoglobulin domain repeats, and a C-terminal GPI-anchor linking it to the membrane.
• It plays roles in T-cell signaling, NK cell cytotoxicity, and immune synapse assembly, as reflected in keywords such as "Immunoglobulin domain", "GPI-anchor", and "Secreted".

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 23-216), suggesting that the antibody likely recognizes the native surface‑exposed epitope of mouse CD48, making it well‑suited for flow cytometry on live cells.
• Consider using this antibody for detecting CD48 expression on mouse immune cells such as T cells, NK cells, and antigen‑presenting cells; validate in the relevant sample system.
• When performing flow cytometry, include appropriate isotype controls and perform titration to optimize the signal‑to‑noise ratio.
• Because CD48 is GPI‑anchored, avoid harsh fixation or permeabilization protocols that may disrupt membrane integrity and compromise epitope accessibility.
• Protect the conjugate from prolonged light exposure and use a compatible filter set (e.g., 450/50 bandpass) for detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted partner for diagnostic manufacturers, academic laboratories, and contract research organizations seeking reliable IVD raw materials. We offer a comprehensive portfolio of validated monoclonal and polyclonal antibodies, target‑specific antibody pairs, recombinant proteins, and ancillary reagents. For research areas such as immunology and cell signaling, our sourcing capabilities cover rare‑specificity raw materials like anti‑CD48 antibodies, enabling seamless integration from early‑stage R&D to clinical validation and scale‑up.