Rabbit anti-Mouse CD48 Monoclonal Antibody for FC - P18181

Core Product Specifications and Parameters

Biological Background: CD48 Function and Localization

• CD48 (BCM1, BLAST-1, SLAMF2, sgp-60) is a GPI-anchored glycoprotein of the SLAM family that regulates immune cell activation.
• It interacts via its N-terminal immunoglobulin domain with receptors CD2 and CD244 (2B4) to modulate T-cell and NK-cell function. Related references: PMID:21278219 PMID:9881969
• CD48 participates in T-cell signaling by associating with CD2 to recruit LCK and LAT to the TCR/CD3 complex, promoting LCK phosphorylation. Related references: PMID:9881969
• It induces phosphorylation of CD244 cytoplasmic ITSMs, initiating cytolytic granule release and immunological synapse formation. Related references: PMID:21278219 PMID:9881969
• The protein localizes to the cell membrane and membrane rafts, with a secreted form also detected.
• Structural features include an immunoglobulin domain, disulfide bonds, and N-linked glycosylation; molecular weight is approximately 27 kDa (27,383 Da by sequence).
• CD48 is essential for directed secretion of perforin and granzymes in T-lymphocytes and NK-cells.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 23-216) of mouse CD48, suggesting recognition of an accessible epitope suitable for flow cytometry.
• For flow cytometry, titrate the antibody to determine the optimal staining concentration for your cell system. Include appropriate isotype controls and viability dyes.
• Since the antibody is directly conjugated to , consider compensation settings in multicolor panels; is excited by the red laser (~633 nm) and emits in the far-red range.
• Validate staining specificity using CD48-negative cells or blocking with recombinant protein if needed.
• Ensure single-cell suspension preparation and avoid fixation that may alter epitopes unless compatibility is established.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to IVD raw materials, including this anti-mouse CD48 monoclonal antibody for immunological and cell signaling studies. Our supply capabilities encompass validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target raw-material sourcing—supporting projects from concept to clinic.