Rabbit anti-Mouse CD48 Monoclonal Antibody for FC - P18181

Core Product Specifications and Parameters

Biological Background: CD48 Function and Localization

• Mouse CD48, also known as BCM1, BLAST-1, SLAMF2, or sgp-60, is a member of the signaling lymphocyte activation molecule (SLAM) family of immunoglobulin-like receptors.
• CD48 serves as a high-affinity ligand for CD2 and 2B4 (CD244), playing a pivotal role in modulating innate and adaptive immune responses. Related references: PMID:21278219 PMID:9881969
• Upon binding to CD2, CD48 facilitates the recruitment of the Src family kinase LCK and the adaptor protein LAT to the T-cell receptor (TCR)/CD3 complex, thereby enhancing LCK phosphorylation and downstream signaling. Related references: PMID:9881969
• CD48 engagement of 2B4 (CD244) triggers phosphorylation of immunoreceptor tyrosine-based switch motifs (ITSMs) in the cytoplasmic tail of CD244, leading to formation of the immunological synapse and directed release of perforin- and granzyme-containing lytic granules from cytotoxic T lymphocytes and natural killer (NK) cells. Related references: PMID:21278219 PMID:9881969
• CD48 is a glycosylphosphatidylinositol (GPI)-anchored protein predominantly found in lipid raft microdomains of the plasma membrane; a soluble ectodomain form is also released by proteolytic shedding or phospholipase cleavage.
• The mature protein contains an N-terminal immunoglobulin V-type domain, an O-glycosylated mucin-like stalk, and a C-terminal GPI anchor; its calculated molecular weight is approximately 27.4 kDa.

Experimental Guidance and Technical Tips

• This antibody is pre-conjugated with ABflo® 594, a bright and stable red fluorescent dye optimized for flow cytometry. Titration is recommended to determine the optimal concentration for your cells and instrument.
• The immunogen spans the entire extracellular domain (aa 23‑216) of mouse CD48, ensuring detection of surface‑expressed protein. Include appropriate isotype controls and Fc receptor blocking steps to reduce background.
• As a GPI‑anchored glycoprotein, CD48 can be released from the cell surface by phosphatidylinositol‑specific phospholipase C (PI‑PLC); if fixation is required, validate staining under your fixation/permeabilization conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated one‑stop partner for IVD raw‑material procurement, providing a wide range of validated monoclonal antibodies, recombinant proteins, and ancillary reagents. Our anti‑mouse CD48 antibody supports immunological and inflammation research, and we offer flexible bulk supply, custom conjugation, and tailored sourcing solutions to streamline your assay development from concept to clinic.