Rabbit anti-Mouse CD47 mAb for Flow Cytometry - Q61735

Core Product Specifications and Parameters

Biological Background: CD47 Function and Localization

• Protein identity: Leukocyte surface antigen CD47 (integrin-associated protein, IAP) is a cell membrane protein encoded by the mouse Cd47 gene, a member of the immunoglobulin superfamily.
• Receptor and signaling roles: Acts as a receptor for thrombospondin-1 (THBS1) and modulates integrin signaling via heterotrimeric G proteins (By similarity).
• Cardiovascular and neural functions: Regulates blood pressure through NO signaling (PMID:20610415), pulmonary endothelin signaling (PMID:27742621), and synaptic plasticity in the hippocampus (By similarity). Related references: PMID:20610415 PMID:27742621
• Immune modulation: Receptor for SIRPA and SIRPG, regulating dendritic cell maturation and T-cell activation; positively modulates FAS-dependent T-cell apoptosis (PMID:15917238, By similarity). Related references: PMID:15917238
• Angiogenesis, aging, and wound healing: Suppresses angiogenesis, contributes to metabolic dysregulation during aging (PMID:32679764), and negatively modulates wound healing in response to THBS1 (PMID:18156939). Related references: PMID:32679764 PMID:18156939
• Stem cell regulation: Inhibits stem cell self-renewal by regulating key pluripotency transcription factors (PMID:23591719). Related references: PMID:23591719
• Subcellular localization and modifications: Located on the cell membrane with a single-pass transmembrane helix; post-translationally modified by glycosylation, phosphorylation, disulfide bonds, and pyrrolidone carboxylic acid; may protect erythrocytes from premature elimination (PMID:10856220). Related references: PMID:10856220

Experimental Guidance and Technical Tips

• The immunogen encompasses the N-terminal extracellular domain (aa 19–135), suggesting the antibody may recognize an extracellular epitope suitable for live-cell surface staining in flow cytometry.
• For FC applications, titrate the antibody to determine optimal staining concentration and include appropriate controls (e.g., CD47-overexpressing or knockout cells, isotype control).
• Since the antibody is directly conjugated to , no secondary detection step is needed; wash cells after staining and analyze immediately. Protect samples from light to prevent fluorophore photobleaching.
• Ensure samples are kept on ice during staining to maintain viability and reduce antigen modulation.
• Validate the antibody in your specific experimental system, as performance may vary depending on cell type and sample preparation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research institutions with a comprehensive portfolio of IVD raw materials, including validated monoclonal antibodies for targets like CD47, which is pivotal in immunology, cell adhesion, and cardiovascular research. Our one-stop sourcing capabilities extend to optimized antibody pairs, bulk ancillary reagents, and rare-target raw materials, streamlining your workflow from concept to clinic. Leverage our expertise to accelerate your assay development with reliable, batch-consistent reagents.