Rabbit anti-Mouse CD47 mAb for FC - Q61735

Core Product Specifications and Parameters

Biological Background: CD47 Function and Localization

• Leukocyte surface antigen CD47 (integrin-associated protein IAP, encoded by Cd47) is an adhesive protein that mediates cell-cell interactions and acts as a receptor for thrombospondin THBS1. Alternative splicing produces multiple isoforms.
• It is a multi-transmembrane immunoglobulin superfamily member localized to the cell membrane, undergoing post-translational modifications including glycosylation, disulfide bond formation, and phosphorylation.
• CD47 plays broad roles in signal transduction, cardiovascular homeostasis, inflammation, apoptosis, angiogenesis, cellular self-renewal, and immunoregulation. Related references: PMID:20610415 PMID:23591719 PMID:27742621
• In response to THBS1, CD47 modulates nitrous oxide signaling, acting as a pressor agent to support blood pressure. Related references: PMID:20610415
• CD47 positively regulates FAS-dependent apoptosis in T-cells, possibly by enhancing FAS clustering. Related references: PMID:15917238
• THBS1 engagement inhibits stem cell self-renewal via CD47, likely through regulation of key stem cell transcription factors. Related references: PMID:23591719
• CD47 suppresses angiogenesis and may be involved in metabolic dysregulation during normal aging. Related references: PMID:32679764
• In THBS1-dependent manner, CD47 negatively modulates wound healing and may prevent premature elimination of red blood cells. Related references: PMID:18156939 PMID:10856220

Experimental Guidance and Technical Tips

• The immunogen covers the extracellular domain (amino acids 19-135) of mouse CD47, suggesting the antibody targets the N-terminal region involved in ligand binding and cell-surface recognition.
• For flow cytometry, optimize antibody concentration by titration on mouse cell lines or primary cells known to express CD47; include appropriate isotype controls to ensure specificity.
• Ensure proper sample preparation (single-cell suspension and Fc receptor blocking) to minimize nonspecific binding, especially when analyzing mouse leukocytes.
• Use directly labeled conjugate to avoid secondary antibody steps, reducing background and simplifying the staining protocol.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with reliable one-stop access to high-quality IVD raw materials. This anti-mouse CD47 monoclonal antibody exemplifies our portfolio covering immunology and inflammation targets. From optimized antibody pairs and conjugates to bulk ancillary reagents, CamelBio streamlines your procurement from concept to clinic, including support for rare-target raw-material sourcing.