Rabbit anti-Mouse CD45.1 Monoclonal Antibody for Flow Cytometry - P06800

Core Product Specifications and Parameters

Biological Background: CD45 Function and Localization

• CD45, encoded by the Ptprc gene in mouse, is a receptor-type tyrosine-protein phosphatase also known as leukocyte common antigen (L-CA), Ly-5, T200, and B220.
• It plays a critical role in T-cell activation by dephosphorylating key signaling molecules such as SKAP1, FYN, and LYN, and modulating T-cell coactivation upon binding to DPP4 and CLEC10A.
• Multiple isoforms are generated by alternative splicing, with apparent molecular weights ranging from 130 to 144 kDa, reflecting differential glycosylation and sequence variations.
• CD45 is localized primarily at the cell membrane, enriched in membrane rafts and at the immunological synapse.
• The protein contains two intracellular phosphatase domains; the first has enzymatic activity, while the second influences substrate specificity.
• Key post-translational modifications include phosphorylation and extensive N-linked glycosylation, which contribute to its structural heterogeneity and functional regulation.

Experimental Guidance and Technical Tips

• This -conjugated monoclonal antibody is recommended for flow cytometry; consider titrating the antibody to determine the optimal concentration for your specific cell population.
• Use appropriate fluorochrome compensation controls and isotype controls to ensure accurate gating and minimize background.
• Validate the antibody in mouse leukocyte samples, such as splenocytes or peripheral blood mononuclear cells, to confirm reactivity and specificity.
• Protect the conjugate from light and store at 2-8°C; avoid freeze-thaw cycles to preserve fluorophore stability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a comprehensive one-stop platform for IVD raw material procurement, from validated antibody pairs to bulk ancillary reagents. For CD45-related immunophenotyping and T-cell activation research, our supply chain ensures consistent access to high-quality monoclonal antibodies, recombinant proteins, and technical support to accelerate your assay development.