Rabbit anti-Mouse CD45.1 Monoclonal Antibody for FC - P06800

Core Product Specifications and Parameters

Biological Background: CD45.1 Function and Localization

• CD45, also known as Leukocyte common antigen (L-CA), Ly-5, or T200, is a receptor-type tyrosine-protein phosphatase C encoded by the Ptprc gene. The CD45.1 allotype is a specific isoform recognized by this antibody.
• Functions as a key regulator of T-cell activation through the antigen receptor, acting as a positive co-stimulatory molecule upon binding to DPP4.
• The intracellular region contains two tandem phosphatase domains; the first domain possesses catalytic activity, while the second modulates substrate specificity.
• Dephosphorylates signaling molecules such as SKAP1, FYN, and LYN, thereby modulating downstream T-cell signaling.
• Localized to the cell membrane, membrane rafts, and immunological synapses, facilitating its role in immune cell communication.
• Undergoes extensive alternative splicing and post-translational modifications including glycosylation and phosphorylation, generating multiple isoforms with distinct extracellular domains.

Experimental Guidance and Technical Tips

• As this antibody is raised against a synthetic peptide within amino acids 311–410 of mouse CD45.1, it is expected to specifically detect the CD45.1 allotype. Verify specificity using CD45.1-positive and -negative cell lines or mouse strains.
• For flow cytometry, titrate the antibody to determine the optimal concentration that provides clear separation between positive and negative populations.
• Include appropriate isotype controls and Fc receptor blocking steps to minimize non-specific binding.
• Consider using this antibody in multicolor panels; ensure fluorophore compensation is properly set.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw material sourcing by offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. This anti-CD45.1 monoclonal antibody supports immunophenotyping and T-cell research applications, with CamelBio providing one-stop access from concept to clinic.