Rabbit anti-Mouse CD45.1 mAb - P06800

Core Product Specifications and Parameters

Biological Background: CD45.1 Function and Localization

• CD45 (Receptor-type tyrosine-protein phosphatase C) is a transmembrane phosphatase encoded by the Ptprc gene in mice. Alternative splicing generates multiple isoforms, including CD45.1, which differ in their extracellular domain length and glycosylation.
• CD45 functions as a positive regulator of T-cell activation by dephosphorylating negative regulatory tyrosine residues on Src-family kinases (e.g., LYN, FYN) and the adapter SKAP1, facilitating TCR signal transduction.
• The intracellular region contains two tandem phosphatase domains; the membrane-proximal D1 domain provides catalytic activity, while the D2 domain modulates substrate specificity.
• Beyond TCR components, CD45 engages with DPP4 to enhance co-stimulation and with CLEC10A on immature dendritic cells to limit autoreactivity, highlighting its role in immune homeostasis.
• It is anchored at the plasma membrane and partitions into lipid rafts; during T-cell activation, CD45 redistributes to the immunological synapse to form signaling microclusters.
• CD45 undergoes extensive N-linked glycosylation and serine/threonine/tyrosine phosphorylation, which regulate its localization, conformation, and enzymatic activity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to an extracellular sequence (aa 311–410) of mouse CD45.1, indicating that the antibody likely recognizes the native membrane-bound form, making it well-suited for surface staining in flow cytometry.
• Since the antibody is pre-conjugated to , it can be directly used in multicolor panels without secondary staining; proper compensation controls are recommended.
• For optimal flow cytometry results, we recommend titrating the antibody on your specific cell population to determine the saturation concentration and minimize background.
• As CD45.1 is broadly expressed on all hematopoietic lineage cells, include appropriate isotype controls and FSC/SSC gating to distinguish leukocyte subsets.
• When staining tissues or cells with high Fc receptor expression, pre-incubation with Fc block is advised to reduce non-specific binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for IVD raw material procurement, offering a comprehensive range of validated antibodies, recombinant proteins, and ancillary reagents. This anti-mouse CD45.1 monoclonal antibody exemplifies our focus on high-quality immune cell markers essential for flow cytometry-based diagnostics and research. Whether you need custom bulk orders, antibody pairs, or support sourcing rare targets, CamelBio streamlines your supply chain from concept to clinic.