Rabbit anti-Mouse CD45 Monoclonal Antibody for IF/ICC, FC - P06800

Core Product Specifications and Parameters

Biological Background: CD45 Function and Localization

• CD45, also known as leukocyte common antigen (L-CA) and Ly-5, is a receptor-type protein tyrosine phosphatase expressed on the surface of all nucleated hematopoietic cells.
• It plays a critical role in T-cell activation by dephosphorylating key signaling molecules such as SKAP1, FYN, and LYN, thereby modulating downstream signaling thresholds.
• Through its interaction with DPP4, CD45 acts as a positive regulator of T-cell coactivation, enhancing antigen receptor signaling.
• At the immunological synapse, CD45 interacts with CLEC10A on dendritic cells, recognizing Tn antigen-modified CD45 on effector T cells to fine-tune activation and limit autoreactivity.
• The protein contains two tandem intracellular phosphatase domains; the first domain possesses catalytic activity, while the second influences substrate specificity.
• Alternative splicing generates multiple isoforms, including B220 (CD45R), which are differentially expressed among leukocyte subsets, enabling diverse functional regulation.
• CD45 localizes to the cell membrane, membrane rafts, and the immunological synapse, consistent with its role in signal initiation and co-receptor modulation.
• Extensive post-translational modifications, including N-linked glycosylation and phosphorylation, regulate its stability, ligand binding, and phosphatase activity.

Experimental Guidance and Technical Tips

• This antibody is raised against a recombinant protein spanning amino acids 26–566 of mouse CD45, encompassing the entire extracellular domain. It is expected to recognize an extracellular epitope, making it suitable for live-cell staining in flow cytometry without permeabilization.
• For immunofluorescence, standard fixation (e.g., 4% PFA) and optional permeabilization can be used. Membrane staining should be observed due to CD45’s transmembrane topology.
• As a direct conjugate, no secondary antibody is required. Optimal titers should be empirically determined for each experimental system to achieve specific staining with minimal background.
• Given CD45’s high expression on leukocytes, this antibody is ideal for identifying and gating immune cell populations in flow cytometry. Include appropriate isotype and fluorescence-minus-one (FMO) controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic developers and laboratories with an integrated supply of IVD raw materials, spanning validated antibody pairs, monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. This -conjugated anti-CD45 monoclonal antibody facilitates leukocyte profiling and T-cell activation research across murine model systems. Reach out to explore how our one-stop procurement platform can streamline your assay development from concept to clinical application.