Rabbit anti-Mouse CD45 Monoclonal Antibody for IF/ICC and FC - P06800

Core Product Specifications and Parameters

Biological Background: CD45 Function and Localization

• CD45 (PTPRC) is a receptor-type tyrosine-protein phosphatase essential for T-cell activation through the antigen receptor, acting as a positive regulator of T-cell coactivation upon DPP4 binding.
• The protein contains two phosphatase domains: the first has intrinsic enzymatic activity, while the second influences the substrate specificity of the first.
• Alternative splicing generates multiple isoforms, including B220, a well‑known marker for B cells and certain activated T‑cell subsets.
• CD45 localizes predominantly to the cell membrane, enriched in membrane rafts and at the immunological synapse, where it modulates signaling.
• Upon T‑cell receptor engagement, CD45 recruits and dephosphorylates downstream effectors such as SKAP1 and FYN, and modulates LYN activity.
• Interaction with CLEC10A on immature dendritic cells at the T‑cell contact site recognizes Tn antigen‑bearing CD45, helping to fine‑tune T‑cell activation thresholds and limit autoreactivity.
• The protein is extensively glycosylated and phosphorylated, and is classified as a single‑pass type I membrane protein with a signal peptide, tandem repeat regions, and a transmembrane helix.

Experimental Guidance and Technical Tips

• The immunogen encompasses the extracellular domain (amino acids 26–566) of mouse CD45, covering the region where isoform‑specific alternative splicing occurs; the antibody may therefore recognize multiple CD45 isoforms.
• For flow cytometry, consider titrating the antibody on a relevant cell population (e.g., mouse splenocytes) to determine optimal signal‑to‑noise. Use appropriate controls (isotype control, fluorescence minus one) to accurately gate CD45‑positive leukocytes.
• In immunofluorescence (IF/ICC), permeabilization may not be required for surface‑stained CD45, but fixation and permeabilization conditions should be optimized for co‑staining intracellular markers. Use a far‑red‑compatible filter set (e.g., 647 nm laser line) for detection.
• Although validated for IF/ICC and FC, the antibody may be suitable for other applications; however, end‑users should validate performance in their specific assay system with the relevant sample type.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to high‑quality IVD raw materials, spanning validated antibody pairs, monoclonal antibodies, bulk ancillary reagents, and custom rare‑target sourcing. This ‑conjugated anti‑mouse CD45 antibody supports immunophenotyping and T‑cell research applications, underpinned by our end‑to‑end supply capabilities. From concept to clinic, CamelBio accelerates your development pipeline with reliable, one‑stop procurement solutions.