Rabbit anti-Mouse CD45 Monoclonal Antibody for Flow Cytometry - P06800

Core Product Specifications and Parameters

Biological Background: CD45 Function and Localization

• CD45, encoded by the Ptprc gene, is a receptor-type tyrosine-protein phosphatase (PTPase) also known as leukocyte common antigen (L-CA), Ly-5, and T200. It is expressed on all nucleated hematopoietic cells.
• The protein is essential for T-cell activation through the antigen receptor, acting as a positive regulator of coactivation upon binding to DPP4.
• CD45 contains two intracellular PTPase domains: the first has catalytic activity, while the second modulates substrate specificity.
• It dephosphorylates key signaling molecules such as SKAP1, FYN, and LYN, thereby regulating T-cell receptor signaling thresholds.
• Localized to the cell membrane, particularly in membrane rafts and at the immunological synapse, facilitating efficient signal transduction.
• Multiple isoforms are generated by alternative splicing, resulting in proteins with molecular weights of approximately 130–144 kDa, which vary among leukocyte subsets.
• Keywords: Alternative splicing, Glycoprotein, Phosphoprotein, Hydrolase, Membrane, Synapse, Transmembrane.

Experimental Guidance and Technical Tips

• This antibody is recommended for flow cytometry (FC) applications. Optimize the concentration by titration using relevant mouse leukocyte samples.
• The immunogen corresponds to the extracellular domain (amino acids 26–566) of mouse CD45, which encompasses regions important for isoform-specific antibody recognition; consider isoform expression in your cell type.
• Use appropriate isotype controls and Fc receptor blocking to minimize nonspecific binding in flow cytometry experiments.
• Since CD45 is highly expressed on all leukocytes, include a viability dye to exclude dead cells for accurate gating.
• For multicolor panels, verify spectral compatibility with the conjugate and other fluorophores.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and researchers a streamlined sourcing solution for IVD raw materials, including validated antibody pairs and optimized monoclonal/polyclonal antibodies for immune cell targets like CD45. Our portfolio supports flow cytometry applications, and we provide technical consulting to accelerate your assay development from concept to clinic. For bulk or customized antibody orders, CamelBio is your trusted partner in securing high-quality reagents.