Rabbit anti-Mouse CD45 Monoclonal Antibody for Flow Cytometry - P06800

Core Product Specifications and Parameters

Biological Background: CD45 Function and Localization

• CD45 is a receptor-type tyrosine-protein phosphatase C, also known as leukocyte common antigen (L-CA), lymphocyte antigen 5 (Ly-5), and T200.
• It plays a critical role in T-cell activation through the antigen receptor, acting as a positive regulator of T-cell coactivation upon binding to DPP4.
• The first of its two intracellular phosphatase domains possesses enzymatic activity, while the second modulates substrate specificity.
• CD45 is predominantly localized at the cell membrane, membrane rafts, and the immunological synapse.
• It interacts with and dephosphorylates key signaling molecules such as SKAP1, FYN, and LYN, modulating downstream signaling pathways.
• Alternative splicing generates multiple isoforms, giving rise to proteins of varying molecular weights consistent with the observed 130 kDa, 140 kDa, and 144 kDa species.
• The protein is heavily glycosylated and undergoes phosphorylation, classifying it as a glycoprotein and phosphoprotein.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular region (amino acids 26–566), suggesting the antibody recognizes an extracellular epitope, making it suitable for live-cell flow cytometry applications.
• For flow cytometry, titrate the antibody to determine the optimal working concentration for your specific cell population.
• When validating specificity, consider the presence of CD45 isoforms with different molecular weights; confirm the expected band pattern in relevant cell lysates if adapting the antibody for Western blotting.
• Use appropriate isotype controls and ensure proper compensation when using the fluorophore in multicolor flow cytometry panels.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For CD45-related immune cell profiling and signaling research, we provide high-quality anti-CD45 reagents and can assist in sourcing rare targets to accelerate your diagnostic development pipeline.