Rabbit anti-Mouse CD45 mAb - P06800

Core Product Specifications and Parameters

Biological Background: CD45 Function and Localization

• CD45, also known as Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen (L-CA), and Ly-5, is a transmembrane glycoprotein expressed exclusively on nucleated hematopoietic cells. It plays a critical role in lymphocyte development and activation.
• The protein contains two intracellular tyrosine phosphatase domains; the first domain exhibits enzymatic activity, while the second regulates substrate specificity. CD45 dephosphorylates Src family kinases such as LYN and FYN, thereby modulating T-cell and B-cell receptor signaling thresholds.
• Alternative splicing generates multiple isoforms (e.g., CD45R, B220) with molecular weights ranging from 130 to 144 kDa, differentially expressed on lymphocyte subsets and developmental stages.
• CD45 is localized to the cell membrane, enriched in membrane rafts, and recruited to the immunological synapse upon T-cell–antigen-presenting cell interaction, where it interacts with molecules like CLEC10A to fine-tune T-cell activation and prevent autoimmunity.
• Through its phosphatase activity, CD45 positively regulates T-cell coactivation upon binding to DPP4 and is essential for efficient antigen receptor signaling.
• Keywords associated with CD45 include alternative splicing, glycoprotein, hydrolase, phosphoprotein, transmembrane, and synapse, reflecting its diverse regulatory roles.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody spans amino acids 26–566 of mouse CD45, covering the extracellular domain and likely recognizing multiple isoforms. Consider verifying isoform specificity in your target cell population.
• This antibody is directly conjugated to , a violet laser-excitable fluorophore compatible with standard flow cytometry panels. Optimize the antibody titration for your specific instrument and sample type to achieve optimal signal-to-noise ratio.
• For flow cytometry (FC) applications, include appropriate isotype controls and viability dyes to exclude dead cells, as CD45 is highly expressed on viable leukocytes but can be shed or internalized in apoptotic cells.
• Cross-reactivity with mouse CD45 is reported; however, if analyzing cells from other species, validate specificity independently.

CamelBio: Your One-Stop Sourcing Bridge

As a comprehensive IVD raw-material supplier, CamelBio supports diagnostic manufacturers and researchers with a wide range of high-quality antibodies, including validated monoclonal antibodies like this anti-CD45 clone. Our portfolio covers bulk ancillary reagents, optimized antibody pairs, and custom sourcing for rare targets, ensuring seamless integration into your assay development. Whether you need to scale up production or require specialized technical consultation, CamelBio bridges the gap from concept to clinic.