Rabbit Anti-Mouse CD44 Monoclonal Antibody () for Flow Cytometry - P15379

Core Product Specifications and Parameters

Biological Background: CD44 Antigen Function and Localization

• CD44 antigen, also known as Ly-24, Pgp-1, HERMES, ECMR-III, or hyaluronate receptor, is a cell-surface glycoprotein encoded by the Cd44 gene (alternative splicing generates multiple isoforms).
• Functions as a receptor for hyaluronan and other extracellular matrix components, mediating cell-cell and cell-matrix adhesion and migration (PubMed:25065622, PubMed:8343954).
• Related references: PMID:25065622 PMID:8343954
• Its cytoplasmic domain assembles signaling complexes with receptor kinases, RhoGTPases (RAC1, RHOA), and phospholipase C, promoting calcium mobilization and actin cytoskeleton reorganization required for cell migration.
• Upon binding to galectin-9 (LGALS9), CD44 activates downstream LCK, ERK, and MAPK pathways, enhancing NK cell activation (PubMed:37006235).
• Related references: PMID:37006235
• Localizes primarily to the plasma membrane and microvilli; a soluble secreted form is also produced via proteolytic cleavage.
• Tissue specificity: Expressed in the intestinal epithelium.
• Post-translational modifications include N- and O-glycosylation, phosphorylation, proteoglycan modification, disulfide bond formation, and sulfation.

Experimental Guidance and Technical Tips

• This antibody is conjugated to , a far-red fluorophore excited by 633–647 nm lasers; use appropriate filter sets (e.g., 710/50 or 730/45) for detection.
• For flow cytometry, titrate the antibody to determine the optimal dilution for your cell type and experimental conditions; a typical starting range is 0.5–5 µl per test.
• Always include appropriate isotype controls and fluorescence-minus-one (FMO) controls to ensure specific staining.
• Protect the antibody conjugate from prolonged light exposure to maintain fluorophore stability.
• Verify CD44 expression in your target cell population (e.g., lymphocytes, activated T cells, or intestinal epithelial cells) using known positive and negative controls.
• The immunogen corresponds to the extracellular domain (aa 23–176) of mouse CD44; thus, the antibody recognizes the N-terminal region and may be used for surface staining of live, unfixed cells.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive procurement partner for IVD raw materials, offering antibody development, bulk manufacturing, and supply chain solutions. For researchers focusing on cell adhesion, immunology, or tumor microenvironment studies, we provide validated monoclonal antibodies like this anti-CD44 clone, along with complementary reagents and rare target sourcing. Our platform bridges the gap between concept and clinic, ensuring reliable access to high-quality raw materials for diagnostic development.