Rabbit Anti-Mouse CD40 Monoclonal Antibody for Flow Cytometry - P27512

Core Product Specifications and Parameters

Biological Background: CD40 Function and Localization

• CD40 (Tumor necrosis factor receptor superfamily member 5) is a cell surface receptor also known as Bp50, B-cell surface antigen CD40, and CD40L receptor.
• Functions as the primary receptor for TNFSF5/CD40LG (CD40 ligand), mediating critical immune signaling pathways (By similarity).
• Transduces TRAF6- and MAP3K8-dependent signals that activate ERK in macrophages and B cells, ultimately triggering immunoglobulin secretion (PubMed:12881420). Related references: PMID:12881420
• Encoded by the Cd40 gene (Tnfrsf5), alternative splicing generates multiple isoforms, including membrane-bound and secreted forms.
• Predominantly localized on the cell membrane, with a secreted isoform also reported; the protein contains a signal peptide, transmembrane helix, and cysteine-rich extracellular repeats.
• Undergoes post-translational modifications including N-linked glycosylation and disulfide bond formation, characteristic of the TNF receptor superfamily.
• Plays a central role in adaptive immunity, particularly in B cell activation, germinal center formation, and antibody class switching.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 20–189 of the mouse CD40 extracellular domain, enabling recognition of the native ligand-binding region.
• This antibody is conjugated to , a bright green-fluorescent dye, making it directly applicable for flow cytometry without secondary staining.
• For flow cytometry, titrate the antibody to determine the optimal staining concentration; include appropriate isotype controls and viability dyes.
• Avoid repeated freeze-thaw cycles to maintain conjugate stability and performance.
• As a directly labeled monoclonal antibody, it facilitates multi-color panel design; however, cross-reactivity with other species has not been tested beyond mouse.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive partner for IVD raw material sourcing, supporting diagnostic manufacturers and research laboratories from early development to clinical application. For researchers investigating CD40-mediated signaling, B cell biology, or immune checkpoint pathways, CamelBio offers validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom raw-material procurement. Our global supply network ensures reliable access to rare-target reagents, helping you accelerate assay development with confidence.