Rabbit anti-Mouse CD40 Monoclonal Antibody for FC - P27512

Core Product Specifications and Parameters

Biological Background: CD40 Function and Localization

• CD40, also known as Bp50 or tumor necrosis factor receptor superfamily member 5, is a cell surface receptor that binds CD40LG, playing a critical role in adaptive immunity.
• Upon ligand binding, CD40 transduces signals through TRAF6 and MAP3K8, activating ERK in macrophages and B cells, which leads to the induction of immunoglobulin secretion.
  Related references: PMID:12881420
• Alternative splicing generates multiple isoforms, including a secreted form; the canonical protein is a type I transmembrane protein localized to the cell membrane.
• CD40 belongs to the tumor necrosis factor receptor superfamily and contains characteristic cysteine-rich repeats in its extracellular domain.
• Post-translational modifications include glycosylation and disulfide bond formation, which are important for structural stability and function.
• The protein is also expressed as a soluble isoform, which may modulate receptor activity in extracellular compartments.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody includes the extracellular domain (amino acids 20-189), which is exposed on the cell surface, making it well-suited for flow cytometry of live cells.
• For flow cytometry, titrate the antibody to determine the optimal concentration for your specific experimental system, using a suitable isotype control to assess non-specific binding.
• Validate the antibody in relevant mouse cell lines or primary cells known to express CD40, such as B lymphocytes or macrophage populations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and researchers with high-quality IVD raw materials, including validated antibodies like this anti-mouse CD40 clone. We offer a comprehensive portfolio of antibody pairs, bulk ancillary reagents, and rare target sourcing services to support your immunology and cell biology workflows. Let us streamline your procurement so you can focus on advancing your diagnostic and research projects.