Rabbit anti-Mouse CD4 Monoclonal Antibody for FC - P06332

Core Product Specifications and Parameters

Biological Background: CD4 Function and Localization

• CD4, also known as L3T4 or Ly-4, is a T-cell surface glycoprotein belonging to the immunoglobulin superfamily. It serves as a critical coreceptor for the T-cell receptor (TCR) by binding to MHC class II molecules on antigen-presenting cells.
• Through its cytoplasmic domain, CD4 recruits the Src kinase LCK to the TCR-CD3 complex, initiating intracellular signaling cascades that drive T-cell activation, lymphokine production, motility, and adhesion.
• CD4 is predominantly a marker of T-helper cells, which orchestrate adaptive immune responses including B-cell regulation and activation of cytotoxic T-cells. It is also expressed on macrophages, dendritic cells, and NK cells, where it modulates differentiation and cytokine expression.
• The protein localizes to the cell membrane via a single-pass transmembrane domain, positioning its extracellular region for ligand interaction and intracellular tail for signaling.
• Post-translational modifications include N-linked glycosylation, disulfide bond formation, palmitoylation, and phosphorylation, which regulate its stability and function.
• Alternative splicing generates distinct isoforms, although the major form in T-cells is the full-length glycoprotein.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 27–394, which encompasses the extracellular domain of mouse CD4. This design favors recognition of the native surface antigen on live cells.
• This antibody is validated for flow cytometry (FC) in mouse samples. Consider performing a titration to determine the optimal concentration for your specific cell type and staining protocol.
• For multicolor flow cytometry panels, evaluate spectral overlap with the fluorophore and include appropriate compensation controls.
• Ensure cell viability and proper Fc receptor blocking to minimize non-specific binding when analyzing primary mouse immune cells.

CamelBio: Your One-Stop Sourcing Bridge

This CD4 antibody, ideal for immunology and immune cell profiling research, is part of CamelBio’s comprehensive IVD raw-material portfolio. CamelBio supports diagnostic manufacturers with validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and access to rare target materials—helping you streamline sourcing from discovery to production.