Rabbit anti-Mouse CD4 Monoclonal Antibody for FC - P06332

Core Product Specifications and Parameters

Biological Background: CD4 Function and Localization

• CD4 is a 51 kDa integral membrane glycoprotein primarily expressed on T-helper cells, where it functions as a coreceptor for MHC class II molecules.
• It plays a crucial role in T-cell activation by simultaneously binding to the T-cell receptor (TCR) and MHC class II-peptide complexes, recruiting the Src kinase LCK to phosphorylate downstream signaling substrates.
• Beyond T cells, CD4 is also present on other immune cells, including macrophages, dendritic cells, and NK cells, where it modulates differentiation, cytokine expression, and cell migration.
• The protein is localized to the cell membrane and undergoes post-translational modifications such as glycosylation, phosphorylation, and palmitoylation, which regulate its function and membrane anchoring.
• CD4 is a key marker for T-helper cells, essential for thymic development and the activation of cytotoxic T cells, B cells, and phagocytes.
• Alternative splicing generates multiple isoforms, and the protein contains immunoglobulin-like domains that mediate its interaction with MHC class II and LCK.

Experimental Guidance and Technical Tips

• The immunogen (recombinant fusion protein covering amino acids 27-394) includes the extracellular domain of mouse CD4, suggesting the antibody likely recognizes the native protein. Validate in mouse lymphocyte populations by flow cytometry.
• For flow cytometry (FC), optimize staining with conjugate using standard protocols for surface staining of live cells. Consider titration to determine the optimal antibody concentration.
• Since this is a rabbit monoclonal antibody, it may exhibit high specificity and low batch-to-batch variability, beneficial for consistent immunophenotyping results.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research laboratories with high-quality IVD raw materials like this anti-mouse CD4 antibody, ideal for T-cell immunophenotyping and immune response studies. We offer validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents to support your assay development, with expertise in sourcing rare targets and custom formulations to bridge your concept-to-clinic needs.