Rabbit anti-Mouse CD4 mAb for FC - P06332

Core Product Specifications and Parameters

Biological Background: CD4 Function and Localization

• CD4 (T-cell surface glycoprotein CD4) is also known as T-cell differentiation antigen L3T4 and T-cell surface antigen T4/Leu-3. It is an integral membrane glycoprotein essential for immune responses.
• Functions as a coreceptor for MHC class II molecules, interacting simultaneously with the T-cell receptor (TCR) and MHC class II/peptide complexes on antigen-presenting cells.
• Recruits the Src kinase LCK to the TCR-CD3 complex, initiating intracellular signaling pathways that lead to T-helper cell activation, lymphokine production, and adhesion.
• In non-T cells such as macrophages and NK cells, CD4 contributes to differentiation, cytokine expression, and cell migration via a TCR/LCK-independent pathway.
• Subcellularly localized to the cell membrane.
• Highly expressed in T-helper cells and also present in macrophages, dendritic cells, and NK cells; CD4 expression is a hallmark of T-helper cells.
• Post-translational modifications include disulfide bond formation, glycosylation, palmitoylation, and phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 27-394 of mouse CD4, encompassing the extracellular immunoglobulin-like domains. This region is critical for MHC class II interaction and may be recognized in native conformation.
• Designed for flow cytometry (FC), this conjugate provides bright fluorescence suitable for multi-color panels. Titration is recommended to optimize signal-to-noise ratio.
• For surface staining, use live, unfixed cells to preserve epitope integrity. Include appropriate isotype and viability controls in experiments.
• Validate performance in your specific cell population, considering that CD4 expression levels can vary among subsets.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a streamlined sourcing experience for diagnostic manufacturers and research laboratories requiring IVD raw materials. This anti-CD4 antibody is part of our immunology portfolio, supporting T-cell research applications. From validated antibody pairs to bulk monoclonal antibodies and ancillary reagents, we offer one-stop procurement solutions to accelerate your projects from concept to clinic.