Rabbit anti-Mouse CD38 Monoclonal Antibody for Flow Cytometry - P56528

Core Product Specifications and Parameters

Biological Background: CD38 Function and Localization

• CD38 is a multifunctional transmembrane glycoprotein with ADP-ribosyl cyclase and cyclic ADP-ribose (cADPR) hydrolase activities, generating cADPR from NAD+.
• It synthesizes the Ca2+ mobilizer nicotinate-adenine dinucleotide phosphate (NAADP+) from 2'-phospho-cADPR and nicotinic acid or from NADP+ and nicotinic acid. Related references: PMID:11829748
• CD38 functions as a receptor for CD31 on endothelial cells, promoting lymphocyte activation, proliferation, and transendothelial migration.
• It regulates dendritic cell functions at the mature stage, including CCL21-driven migration, survival, and Th1-polarizing activity.
• In lamina propria T lymphocytes, CD38/CD31 interactions trigger LCK and LAT signaling cascades leading to cytokine release.
• The protein is a membrane-embedded enzyme with a predicted molecular weight of ~34 kDa, containing a signal-anchor, transmembrane helix, and disulfide bonds, and is glycosylated.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 45–304); the antibody likely recognizes a surface epitope and is suitable for live-cell flow cytometry.
• The antibody is pre-conjugated with , eliminating secondary staining steps; titrate to determine optimal staining concentration.
• Specificity for mouse CD38 is reported; cross-reactivity to other species is not known, so validate if used in non-mouse systems.
• Include appropriate isotype controls and viability dyes to gate on live, single cells and assess non-specific binding.
• CD38 is expressed on various immune cells; consider co-staining with lineage markers for subset analysis.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive range of IVD raw materials, from validated antibody pairs and optimized monoclonal/polyclonal antibodies to bulk ancillary reagents. This anti-mouse CD38 monoclonal antibody, targeting a key immune regulator, complements our immunology and inflammatory disease portfolio. For custom sourcing of rare targets or large-volume procurement, our expert team offers end-to-end support from concept to clinic.