Rabbit anti-Mouse CD38 Monoclonal Antibody for Flow Cytometry - P56528

Core Product Specifications and Parameters

Biological Background: CD38 Function and Localization

• CD38 (also known as I-19, ADPRC-1) is a multifunctional type II transmembrane glycoprotein belonging to the ADP-ribosyl cyclase family.
• It catalyzes the synthesis of cyclic ADP-ribose (cADPR) from NAD+, a potent second messenger that mobilizes intracellular calcium stores.
• CD38 also synthesizes nicotinic acid adenine dinucleotide phosphate (NAADP+) from 2'-phospho-cADPR and nicotinic acid, or from NADP+, a reaction critical for calcium signaling.
  Related references:
  PMID:11829748
• The protein displays cADPR hydrolase activity, converting cADPR to ADP-ribose, thereby regulating cADPR levels.
• As a receptor, CD38 binds to CD31 (PECAM-1) on endothelial cells, promoting lymphocyte activation, proliferation, and transendothelial migration.
• In lamina propria T cells, CD38-CD31 engagement triggers LCK and LAT phosphorylation, leading to cytokine secretion.
• CD38 influences dendritic cell functions, including migration toward CCL21, survival, and Th1 polarization.
• Subcellularly, CD38 localizes to the plasma membrane with a single transmembrane helix and is post-translationally modified by disulfide bonds and glycosylation.

Experimental Guidance and Technical Tips

• The immunogen (aa 45-304) covers the majority of the extracellular domain of mouse CD38, suggesting that the antibody likely recognizes the native surface protein.
• For flow cytometry, titrate the antibody on a relevant mouse cell line or primary cells known to express CD38, such as splenocytes or activated lymphocytes, to determine the optimal staining concentration.
• Include appropriate isotype controls and live/dead discrimination to accurately identify CD38-positive populations.
• Consider co-staining with lineage markers (e.g., CD3, B220, CD11c) to profile CD38 expression across immune subsets.
• Validate specificity using CD38 knockout models or blocking peptides when available.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive pipeline of IVD raw materials, including validated conjugated antibodies, optimized monoclonal and polyclonal reagents, and bulk ancillary products. This anti-mouse CD38 antibody exemplifies our commitment to supporting immune cell profiling and receptor signaling research. Beyond catalog products, we provide customized sourcing for rare targets and end-to-end support from concept to clinical application.