Rabbit anti-Mouse CD352/SLAMF6 Monoclonal Antibody for Flow Cytometry - Q9ET39

Core Product Specifications and Parameters

Biological Background: CD352/SLAMF6 Function and Localization

• SLAMF6 (signaling lymphocytic activation molecule family member 6), also designated CD352, Ly108, NTB-A, KAL1, and SF2000, is a type I transmembrane receptor belonging to the SLAM family. It contains extracellular immunoglobulin-like domains and cytoplasmic immunoreceptor tyrosine-based switch motifs (ITSMs) that mediate signal transduction. Alternative splicing yields multiple isoforms with distinct functional properties.
• SLAMF6 acts as a self-ligand receptor whose signaling outcomes depend on the presence of small cytoplasmic adapters SH2D1A/SAP and SH2D1B/EAT-2, thereby modulating the activation and differentiation of a wide variety of immune cells in both innate and adaptive immunity. Related references: PMID:19648922
• In natural killer (NK) cells, SLAMF6 triggers cytolytic activity only when high surface densities of natural cytotoxicity receptors are present; this positive signaling involves VAV1 phosphorylation and depends on SH2D1B.
• In conjunction with SLAMF1, SLAMF6 controls the transition between positive selection and the subsequent expansion and differentiation of thymic natural killer T (NKT) cells. Related references: PMID:18031695
• Together with SLAMF1 and CD84/SLAMF5, SLAMF6 may negatively regulate the humoral immune response. It also negatively regulates germinal center formation by inhibiting T-cell:B-cell adhesion, a process involving increased association with PTPN6/SHP-1 in the absence of SH2D1A/SAP. Related references: PMID:25926831, PMID:22683125
• SLAMF6 mediates T-cell adhesion, participates in stable T-cell:B-cell interactions, and is crucial for maintaining B-cell tolerance in germinal centers and preventing autoimmunity. Isoform 3 may act as a suppressor of pathogenic T-cell proliferation. Related references: PMID:20153220, PMID:25801429, PMID:21422172
• Subcellular localization: Cell membrane.
• Tissue specificity: Expressed on hematopoietic cells; isoform 3 is expressed in thymocytes and B lymphocytes of the C57Bl/6 strain.
• Post-translational modifications include glycosylation, phosphorylation, and disulfide bond formation. The protein contains a signal peptide and an immunoglobulin-like domain.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular region (amino acids 31-235) of mouse CD352/SLAMF6, suggesting the antibody recognizes an extracellular epitope and is suitable for flow cytometry on live cells.
• For flow cytometry, titrate the antibody to determine the optimal concentration for your specific cell type and staining conditions; a starting range of 0.1-10 µg/mL is typical for conjugated antibodies.
• Include appropriate controls: use isotype-matched control antibody conjugates and unstained cells to set gates and assess nonspecific binding.
• Validate the antibody in your experimental system using known SLAMF6-expressing cells (e.g., lymphocytes) as positive controls and SLAMF6-negative cells as negative controls.
• Consider that SLAMF6 expression may vary with activation status; stimulation with cytokines or antigens can modulate surface levels.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents to support your diagnostic development from concept to clinic. This anti-mouse CD352/SLAMF6 monoclonal antibody, targeting a key immune receptor involved in NK cell and T-cell regulation, exemplifies our commitment to providing high-quality, application-validated reagents for immunology research. In addition to catalog products, CamelBio offers tailored solutions for rare target sourcing and technical consulting, ensuring you have the tools needed to advance your assay development efficiently.