Rabbit anti-Mouse CD34 Monoclonal Antibody for Flow Cytometry - Q64314

Core Product Specifications and Parameters

Biological Background: Hematopoietic Progenitor Cell Antigen CD34 Function and Localization

• CD34 is a single-pass transmembrane glycoprotein that belongs to the CD34 family; it undergoes alternative splicing to produce multiple isoforms.
• The protein is thought to mediate stem cell attachment to the bone marrow extracellular matrix or directly to stromal cells, playing a critical role in early hematopoiesis.
• CD34 presents carbohydrate ligands to selectins, facilitating stem cell binding to lectins expressed by stromal cells or other marrow components (by similarity).
• It is predominantly localized to the cell membrane.
• In mouse, CD34 mRNA is highly expressed in hematopoietic progenitor cell lines, brain, and testis, and moderately in thymus, spleen, and bone marrow; it is absent in adult liver (PubMed:1709048). At the protein level, CD34 is detected in the kidney, specifically in the thin limb of Henle’s loop (PubMed:31605441). Related references: PMID:1709048 PMID:31605441
• Post-translational modifications include glycosylation and phosphorylation, as indicated by the keywords glycoprotein and phosphoprotein.

Experimental Guidance and Technical Tips

• The immunogen spans the predicted extracellular region (aa 35-287) of mouse CD34, making it suitable for detecting surface-expressed CD34 in flow cytometry without permeabilization.
• For flow cytometry, titrate the antibody to determine the optimal concentration for your specific cell type and include appropriate isotype controls and viability dyes.
• Given the expected expression in hematopoietic progenitors, prepare single-cell suspensions from bone marrow or spleen carefully and consider lineage-specific gating strategies.
• Validate staining in the relevant sample system, as expression levels can vary across tissues and developmental stages.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers with a comprehensive portfolio of IVD raw materials, including validated monoclonal antibodies, antibody pairs, and ancillary reagents. This anti-mouse CD34 antibody is part of our offering for hematopoietic and stem cell research applications, and we can assist with bulk supply, custom conjugation, and rare target sourcing. Contact us to streamline your raw material procurement from concept to clinic.