Rabbit anti-Mouse CD34 Monoclonal Antibody for FC - Q64314

Core Product Specifications and Parameters

Biological Background: CD34 Function and Localization

• CD34, also known as hematopoietic progenitor cell antigen CD34, is a cell surface glycoprotein involved in cell adhesion and early hematopoiesis.
• It functions as a potential adhesion molecule that mediates stem cell attachment to bone marrow extracellular matrix or stromal cells, possibly by presenting carbohydrate ligands to selectins (By similarity).
• The protein is localized to the membrane, consistent with its role in cell-cell and cell-matrix interactions.
• Expression is highest in hematopoietic progenitor cell lines, brain, and testis, with moderate levels in thymus, spleen, and bone marrow, but absent from adult liver (PubMed:1709048).
• At the protein level, CD34 is detected in the kidney, specifically in the thin limb of Henle's loop (PubMed:31605441).
• Post-translational modifications include phosphorylation (as indicated by keyword "Phosphoprotein"), and it is predicted to contain a signal peptide and a transmembrane helix.
• The gene undergoes alternative splicing, suggesting potential isoforms (keyword "Alternative splicing"), though the functional significance is unclear.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 35-287), targeting the mature protein after signal peptide cleavage. For flow cytometry, consider titrating the antibody to optimal concentration for your cell type and experimental conditions.
• Cross-reactivity is reported for mouse. For other species, verify sequence homology before use.
• Ensure the antibody conjugate is compatible with your flow cytometry panel; avoid spectral overlap with other fluorophores.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, from monoclonal antibodies to ancillary reagents. For CD34-related hematopoietic research, we offer validated antibody pairs, bulk antibodies, and rare target sourcing to accelerate your assay development and clinical transition. Our supply chain expertise ensures stable, high-quality raw materials for every stage of your workflow.