Rabbit anti-Mouse CD34 mAb - Q64314

Core Product Specifications and Parameters

Biological Background: CD34 Function and Localization

• CD34 is a hematopoietic progenitor cell antigen and a member of the CD34 family, also known by aliases GIG3 and MORT1.
• The protein is a single-pass type I membrane glycoprotein that functions as a cell adhesion molecule, potentially mediating the attachment of stem cells to the bone marrow extracellular matrix or stromal cells.
• It presents carbohydrate ligands to selectins, and may serve as a scaffold for lineage-specific glycan attachment, facilitating lectin-mediated interactions during early hematopoiesis.
• CD34 undergoes alternative splicing, yielding isoforms with molecular weights around 35-41 kDa.
• Highly expressed in hematopoietic progenitor cell lines, brain, and testis, with moderate expression in thymus, spleen, and bone marrow; not detected in adult liver. Related references: PMID:1709048
• At the protein level, CD34 is detected in the kidney, specifically in the thin limb of Henle's loop. Related references: PMID:31605441
• Key post-translational modifications include glycosylation and phosphorylation, as indicated by glycoprotein and phosphoprotein keywords.
• Contains a signal peptide and a transmembrane helix, anchoring it to the cell membrane.

Experimental Guidance and Technical Tips

• For IF/ICC, consider optimizing fixation and permeabilization protocols to preserve membrane antigen accessibility and achieve clear cell surface staining.
• In flow cytometry, titrate the conjugate to determine the optimal concentration for mouse cell suspensions, and include appropriate isotype controls.
• Since the immunogen spans the extracellular domain (aa 35-287), the antibody likely recognizes native surface CD34; validate specificity using CD34-negative cells or knockout models.
• Note that the UniProt-calculated molecular weight is ~40 kDa, but observed isoforms may range from 35 to 41 kDa; use protein markers accordingly.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents. This anti-mouse CD34 monoclonal antibody is designed for hematopoietic stem cell research and flow cytometry-based diagnostic applications. For customizable or rare target raw material sourcing, our team provides tailored procurement solutions to accelerate your assay development from concept to clinic.