Rabbit anti-Mouse CD317/BST2 Monoclonal Antibody for Flow Cytometry - Q8R2Q8

Core Product Specifications and Parameters

Biological Background: CD317/BST2 Function and Localization

• CD317, also known as bone marrow stromal antigen 2 (BST2), HM1.24 antigen, or tetherin, is an IFN-induced antiviral restriction factor encoded by the Bst2 gene in mouse.
• BST2 directly blocks the release of diverse enveloped viruses from infected cells by tethering nascent virions to the cell membrane via its unique topology—a transmembrane domain and a GPI anchor.
• Targeted viruses include retroviruses (HIV-1, MLV, MMTV), filoviruses (Ebola virus), arenaviruses (Lassa virus), and rhabdoviruses (VSV); tethered virions may be internalized and degraded.
• BST2 can inhibit cell surface proteolytic activity of MMP14, leading to reduced MMP15 activation and subsequently suppressing cell growth and migration.
• BST2 stimulates signaling through LILRA4/ILT7 on plasmacytoid dendritic cells, providing negative feedback to type I IFN production in response to viral infection.
• It plays a role in organizing the subapical actin cytoskeleton in polarized epithelial cells.
• Subcellularly, BST2 localizes to the trans-Golgi network, cell membrane (including membrane rafts and apical membrane), late endosomes, and cytoplasm.
• In naïve mice, BST2 is specifically expressed at the protein level on type I interferon-producing cells, particularly plasmacytoid dendritic cells.

Experimental Guidance and Technical Tips

• The immunogen encompasses the extracellular domain (aa 55-152) of mouse BST2, making this -conjugated antibody suitable for surface staining in live-cell flow cytometry applications.
• For flow cytometry, titrate the antibody to determine the optimal working dilution for your specific cell type and always include appropriate isotype control and fluorescence-minus-one (FMO) controls.
• BST2 expression is known to be induced by type I IFNs; thus, consider stimulating cells with IFN-α/β to enhance signal when detecting endogenous BST2 in certain cell types.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to high-quality IVD raw materials, including validated antibody pairs, recombinant proteins, and conjugated monoclonal antibodies like this anti-CD317/BST2 clone. With expertise in innate immunity and antiviral restriction factor targets, we offer tailored supply solutions for flow cytometry and immunoassay development. As your one-stop partner, we support every stage from early R&D to scalable clinical reagent delivery, reducing sourcing complexity and accelerating your project timelines.