Rabbit anti-Mouse CD301 (MGL1/2) Monoclonal Antibody for Flow Cytometry - P49300 / Q8JZN1

Core Product Specifications and Parameters

Biological Background: CD301 (MGL1/2) Function and Localization

• CD301, also known as MGL1/2, consists of two closely related C-type lectin receptors in mice: CD301a (encoded by Clec10a/Mgl1) and CD301b (encoded by Clec10b/Mgl2). CD301a is a type II transmembrane glycoprotein with a single carbohydrate recognition domain (CRD) that specifically binds terminal galactose and N-acetylgalactosamine (GalNAc) residues on glycoproteins.
• CD301a plays a key role in macrophage-mediated recognition of aberrant glycosylation on tumor cells, facilitating interactions between tumoricidal macrophages and tumor cells.
• Studies in diet-induced obesity models have shown that CD301a contributes to the recruitment of inflammatory monocytes to adipose tissue, impacting metabolic inflammation. Related references: PMID:19995956
• In infectious disease, CD301a is critical for resistance to Trypanosoma cruzi infection by ensuring optimal activation of macrophages. Related references: PMID:25170304
• Subcellular localization is predominantly at the plasma membrane, consistent with its role as a cell-surface receptor.
• Tissue expression includes lymph node (subcapsular sinus, interfollicular areas), as well as heart, lung, testis, skeletal muscle, spleen, brain, kidney, and thymus; at the cellular level, it is detected on activated macrophages and in macrophage cell lines (e.g., RAW 264.7).
• The protein contains conserved structural features such as calcium-dependent carbohydrate-binding activity, disulfide bonds within the CRD, and N-linked glycosylation sites.

Experimental Guidance and Technical Tips

• The immunogen consists of a recombinant fusion protein spanning amino acids 57-304 of mouse CD301a, covering the extracellular CRD. When using this conjugate for flow cytometry, consider titrating the antibody to determine optimal staining concentrations for your specific cell population.
• Given the expression on activated macrophages, include appropriate positive controls such as stimulated RAW 264.7 cells or mouse thioglycollate-elicited peritoneal macrophages, and validate specificity using Clec10a-knockout cells or isotype controls.
• For multicolor flow cytometry panels, confirm that the fluorophore is compatible with your instrument's laser/filter configuration and perform compensation controls.
• Because CD301a recognizes glycan ligands, ensure that cell preparation methods do not alter surface glycosylation patterns.

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