Rabbit anti-Mouse CD301 (MGL1/2) Monoclonal Antibody for Flow Cytometry - P49300 / Q8JZN1

Core Product Specifications and Parameters

Biological Background: CD301 (MGL1/2) Function and Localization

• CD301, also known as macrophage galactose-type lectin (MGL), is encoded by the Clec10a gene in mice and exists as two closely related isoforms, CD301a (MGL1) and CD301b (MGL2), which are type II transmembrane C-type lectins.
• It specifically recognizes terminal galactose and N-acetylgalactosamine residues on glycoconjugates, mediating macrophage interactions with pathogens and tumor cells through carbohydrate recognition.
• CD301a plays a role in the recruitment of inflammatory monocytes to adipose tissue in diet-induced obesity, contributing to metabolic inflammation. Related references: PMID:19995956
• It is involved in resistance to Trypanosoma cruzi infection by ensuring optimal activation of macrophages for parasite clearance. Related references: PMID:25170304
• Subcellular localization is primarily on the cell membrane of activated macrophages, with expression detected in lymph node subcapsular sinus, interfollicular regions, and high endothelial venules.
• Tissue expression is broad, including heart, lung, testis, skeletal muscle, spleen, brain, kidney, and thymus, as well as macrophage cell lines like RAW 264.7.
• Keywords associated with CD301a include calcium-dependent lectin, disulfide bond, glycoprotein, signal-anchor, and transmembrane helix, reflecting its structural features.

Experimental Guidance and Technical Tips

• The immunogen was generated using a recombinant protein spanning amino acids 57-304 of mouse CD301a, which corresponds to the extracellular carbohydrate recognition domain, suggesting the antibody may bind native surface-expressed antigen.
• For flow cytometry, consider titrating the antibody to determine the optimal concentration for your specific cell type and include appropriate isotype controls.
• Validate antibody specificity in the relevant mouse model or cell line, as cross-reactivity with other species has not been confirmed.
• Ensure samples are kept on ice or at 2-8°C during staining to maintain cell viability and minimize antigen modulation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw material procurement for diagnostic developers and researchers, offering this anti-CD301 antibody as part of a comprehensive catalog tailored for macrophage and immune function studies. We supply a range of validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and provide sourcing support for rare target raw materials to accelerate your assay development from concept to clinic. Contact us to explore how our one-stop platform can meet your specific project needs.