Rabbit anti-Mouse CD301 (MGL1/2) Monoclonal Antibody for FC - P49300 / Q8JZN1

Core Product Specifications and Parameters

Biological Background: CD301/MGL (Clec10a & Clec10b) Function and Localization

• The CD301 antigen encompasses the macrophage galactose-type lectin family members MGL1 (Clec10a) and MGL2 (Clec10b). This antibody recognizes both MGL1 and MGL2, as evidenced by the dual SWISS accession (P49300/Q8JZN1).
• Clec10a (MGL1) is a C-type lectin receptor that specifically recognizes terminal galactose and N-acetylgalactosamine units, implicating it in glycan-mediated cell recognition pathways.
• May participate in macrophage–tumor cell interactions, potentially contributing to tumoricidal activity.
• Plays a role in the recruitment of inflammatory monocytes to adipose tissue during diet-induced obesity, linking innate immunity to metabolic inflammation. Related references: PMID:19995956
• Contributes to resistance against Trypanosoma cruzi infection by ensuring optimal macrophage activation. Related references: PMID:25170304
• Localizes to the membrane as a single-pass transmembrane protein, with a signal-anchor topology.
• Expressed predominantly on activated macrophages and detected in lymphoid tissues (lymph node subcapsular sinus, interfollicular regions) as well as in heart, lung, testis, skeletal muscle, spleen, brain, kidney, and thymus.
• Post-translational modifications include glycosylation and disulfide bond formation; calcium is required for lectin activity.

Experimental Guidance and Technical Tips

• As the antibody is directly conjugated to (a red fluorescent dye with excitation/emission similar to Alexa Fluor 594), it is ready-to-use for flow cytometry without secondary antibody incubation. Verify optimal working concentration by titration in your experimental system.
• The immunogen corresponds to the extracellular domain of mouse CD301a (amino acids 57–304), suggesting recognition of native surface epitopes. Confirm reactivity in your cellular model, such as activated macrophages or macrophage cell lines (e.g., RAW 264.7).
• For dual-color FC experiments, consider spectral overlap with other fluorophores and use appropriate compensation controls.
• The antibody detects both MGL1 and MGL2; validate specificity using Clec10a or Clec10b knockout/knockdown controls if needed.
• Avoid freeze-thaw cycles; store protected from light to maintain conjugate stability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a one-stop sourcing platform for validated IVD raw materials, including rare-target monoclonal antibodies and ancillary reagents. This product targets macrophage lectins involved in immunity and metabolism, fitting into broader research on infectious disease, cancer, and metabolic disorders. Beyond research-grade antibodies, CamelBio supplies validated antibody pairs, bulk recombinant proteins, and custom raw-material sourcing to support diagnostic development from concept to clinic.