Rabbit anti-Mouse CD28 Monoclonal Antibody for FC - P31041

Core Product Specifications and Parameters

Biological Background: CD28 Function and Localization

• CD28 is a T-cell-specific surface glycoprotein that plays a critical role in T-cell activation, proliferation, survival, and the maintenance of immune homeostasis. Related references: PMID:7688139
• Functions as a costimulatory receptor, amplifying TCR signals and delivering unique signals that alter the gene expression program of T-cells.
• Engagement by its ligands CD80 or CD86 enhances proliferation and cytokine production, particularly IL-2, in both CD4+ and CD8+ T-cell subsets.
• Ligation induces recruitment of protein kinase C-theta (PRKCQ) and GRB2, leading to NF-kappa-B activation via both PI3K/Akt-dependent and -independent pathways.
• In conjunction with TCR/CD3 ligation and CD40L costimulation, CD28 enhances the production of IL-4 and IL-10 in T-cells.
• Contains an immunoglobulin domain, disulfide bonds, and is a glycoprotein, with phosphorylation sites that regulate its activity.
• Localizes to the cell membrane as a single-pass type I membrane protein.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant protein covering amino acids 20–149 of mouse CD28. Consider that the antibody likely recognizes epitopes within this extracellular domain.
• This antibody is validated for flow cytometry (FC) and is provided as a ready-to-use conjugate. For optimal results, titrate the antibody in your specific cell system and include appropriate isotype controls.
• Due to the target's role in T-cell biology, it is recommended to validate performance in relevant mouse T-cell models or primary cells.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive range of IVD raw materials, including this Rabbit anti-Mouse CD28 monoclonal antibody, ideal for flow cytometric analysis of T-cell activation. Our portfolio encompasses validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to support your assay development from concept to clinic.