Rabbit anti-Mouse CD274/PD-L1 Monoclonal Antibody for Flow Cytometry - Q9EP73

Core Product Specifications and Parameters

Biological Background: CD274/PD-L1 Function and Localization

• CD274 (PD-L1), also known as B7-H1, is a type I transmembrane protein belonging to the immunoglobulin superfamily.
• As a ligand for the inhibitory receptor PDCD1 (PD-1), it plays a critical role in maintaining immune tolerance by limiting T-cell effector responses. Related references: PMID:11238124
• Through a yet unidentified activating receptor, CD274 may also costimulate T-cell subsets that predominantly produce IL-10. Related references: PMID:11015443 PMID:12719480
• In the tumor microenvironment, the PD-1/PD-L1 pathway is exploited to attenuate anti-tumor immunity, inhibiting CTL effector function and promoting tumor survival. Related references: PMID:12218188 PMID:27281199
• CD274 localizes to the cell membrane and also cycles through early and recycling endosome membranes.
• It is highly expressed in heart, thymus, skeletal muscle, and lung, and on activated dendritic cells, B cells, and macrophages; it is also found on many tumor cell lines of lymphoid origin.
• Structural features include an immunoglobulin V-like domain, a transmembrane helix, disulfide bonds, and N-linked glycosylation; it may also undergo ubiquitination.
• Functionally, it is involved in adaptive immunity and acts as a receptor that modulates immune responses.

Experimental Guidance and Technical Tips

• The immunogen (aa 19-235 of mouse CD274/PD-L1) corresponds to the extracellular domain, which is predicted to generate antibodies recognizing the native cell surface protein in flow cytometry.
• As a pre-conjugated antibody, it allows direct detection without secondary staining; titrate the antibody for optimal signal-to-noise ratio in your specific experimental system.
• Include appropriate viability dyes to exclude dead cells, as non-specific binding can occur.
• Validate the staining pattern using known positive controls (e.g., activated mouse T cells, dendritic cells, or PD-L1-expressing tumor cell lines) and an isotype control.
• For intracellular staining or detection in permeabilized cells, verify that the epitope is preserved under fixation/permeabilization conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research institutions integrated access to IVD raw materials. This anti-mouse CD274 (PD-L1) product supports immune checkpoint and tumor immunity research applications. With a comprehensive portfolio including validated antibody pairs, recombinant proteins, and ancillary reagents, CamelBio streamlines your sourcing needs from concept to commercialization.