Rabbit anti-Mouse CD272/BTLA mAb for Flow Cytometry - Q7TSA3

Core Product Specifications and Parameters

Biological Background: CD272/BTLA Function and Localization

• CD272/BTLA (B- and T-lymphocyte attenuator) is a type I transmembrane glycoprotein and member of the immunoglobulin superfamily, encoded by the Btla gene.
• It functions as an inhibitory receptor on lymphocytes, downregulating antigen receptor signaling by recruiting protein tyrosine phosphatases SHP-1 (PTPN6) and SHP-2 (PTPN11). Related references: PMID:12796776 PMID:14652006
• BTLA interacts with TNFRSF14 (HVEM) in both cis and trans configurations; cis interactions maintain naive T cell resting state, while trans interactions provide survival signals to effector T cells during adaptive responses. Related references: PMID:19915044
• The protein is expressed on the surface of T cells, B cells, macrophages, NK cells, and dendritic cells, with polymorphic tissue distribution among mouse strains.
• BTLA undergoes post-translational modifications including glycosylation and phosphorylation, and contains a conserved disulfide bond within its extracellular immunoglobulin domain.
• Alternative splicing of the Btla gene produces multiple isoforms, contributing to functional diversity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 30–176), making this antibody suitable for detecting surface BTLA in live-cell flow cytometry without permeabilization.
• For optimal flow cytometry results, titrate the antibody and use appropriate isotype controls, Fc receptor blocking, and viability dyes to reduce nonspecific binding.
• Ensure your flow cytometer’s laser and filter configuration is compatible with (excitation 488 nm, emission ~519 nm).
• Validate antibody performance in relevant mouse immune cell subsets (e.g., splenocytes, lymph node cells) where BTLA is known to be expressed.
• Consider co-staining with lineage markers to analyze BTLA expression on specific lymphocyte populations.

CamelBio: Your One-Stop Sourcing Bridge

With a focus on the BTLA immune checkpoint target, CamelBio serves as a comprehensive IVD raw-material sourcing partner, offering validated antibody pairs, optimized monoclonal antibodies, bulk ancillary reagents, and rare target procurement. This anti-BTLA antibody supports immune-oncology diagnostic development, backed by CamelBio’s supply chain and technical support from concept to clinical application.