Rabbit anti-Mouse CD268/BAFF-R Monoclonal Antibody for Flow Cytometry - Q9D8D0

Core Product Specifications and Parameters

Biological Background: BAFF-R Function and Localization

• BAFF-R (Tumor necrosis factor receptor superfamily member 13C) is a B-cell receptor specific for TNFSF13B/BAFF/BLyS; ligand binding promotes survival of mature B-cells and sustains the B-cell response.
• Encoded by the Tnfrsf13c gene, this receptor is also known by aliases Bcmd, Baffr, and Br3, highlighting its critical role in B-cell maturation and humoral immunity.
• BAFF-R is a single-pass transmembrane protein with a signal-anchor topology; its molecular weight is approximately 18.8 kDa, with glycosylation contributing to the observed 19 kDa form.
• The receptor is highly expressed in spleen and testis, with lower levels in lung and thymus, consistent with its central role in B-cell homeostasis and adaptive immunity.
• Includes disulfide bonds and potential glycosylation sites; categorized under adaptive immunity, alternative splicing, and reference proteome keywords.

Experimental Guidance and Technical Tips

• This -conjugated rabbit monoclonal antibody is recommended for flow cytometry (FC) applications on mouse samples; the fluorophore enables direct detection without secondary reagents.
• The immunogen spans amino acids 10–71 of the mouse CD268/BAFF-R extracellular region; surface staining of live B cells is likely effective, but fixation and permeabilization conditions should be optimized empirically.
• Consider using fresh or properly cryopreserved mouse splenocytes or lymphoid tissues for optimal detection, as BAFF-R expression is prominent in mature B cells.
• Titrate the antibody to determine the optimal staining concentration for your specific experimental setup; inclusion of appropriate isotype controls and Fc receptor blocking is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with streamlined access to high-quality IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For B-cell and immunology studies, we offer sourcing support for rare-target antibodies such as anti-mouse CD268/BAFF-R clones, along with expert technical consulting to accelerate your assay development from concept to clinic.