Rabbit anti-Mouse CD262/TRAILR2 Monoclonal Antibody for FC - Q9QZM4

Core Product Specifications and Parameters

Biological Background: Tumor Necrosis Factor Receptor Superfamily Member 10B Function and Localization

• Protein Identity: Tumor necrosis factor receptor superfamily member 10B (TNFRSF10B), also known as Death receptor 5 (DR5), CD262, TRAILR2, KILLER, TRICKB, and MK. It is a member of the TNFR superfamily.
• Apoptotic Function: Functions as a receptor for the cytotoxic ligand TNFSF10/TRAIL. Upon ligand binding, the adaptor molecule FADD recruits caspase-8 to the receptor, forming the death-inducing signaling complex (DISC), which proteolytically activates caspase-8 to initiate apoptosis via downstream caspases.
• Non-Apoptotic Signaling: Promotes the activation of NF-kappa-B, linking TRAIL signaling to pro-inflammatory and survival pathways.
• ER Stress Response: Essential for endoplasmic reticulum (ER) stress-induced apoptosis, thereby integrating cellular stress signals with the extrinsic death pathway.
• Subcellular Localization: Predominantly localized to the cell membrane, consistent with its role as a transmembrane receptor.
• Tissue Specificity: Highly expressed in heart, lung, and kidney, indicating potential tissue-specific regulation of TRAIL sensitivity.
• Structural Features: Contains characteristic TNFR cysteine-rich repeats, disulfide bonds, and glycosylation sites; possesses a signal peptide and a transmembrane helix, consistent with a type I transmembrane glycoprotein.

Experimental Guidance and Technical Tips

• This antibody is intended for flow cytometry (FC). Because the immunogen corresponds to amino acids 53–170 of the extracellular domain, it is expected to recognize the native surface receptor on mouse cells.
• For optimal flow cytometry staining, consider titrating the antibody to determine the saturating concentration and use appropriate isotype controls and live/dead exclusion dyes.
• Since TNFRSF10B expression can be modulated by cellular stress or TRAIL stimulation, validate staining conditions in the relevant cell lines or primary cells treated under controlled experimental parameters.
• For intracellular staining or for studying receptor internalization, additional permeabilization steps may be required; confirm compatibility with the fluorophore conjugate.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to IVD raw materials, technical services, and consulting—supporting you from concept to clinic. This anti-mouse CD262/TRAILR2 (DR5) monoclonal antibody is a valuable tool for apoptosis and TRAIL signaling research, and CamelBio can also supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to streamline your assay development. Partner with us to bridge your research to reliable diagnostic solutions.