Rabbit anti-Mouse CD252 (OX40 Ligand) Monoclonal Antibody for IF/ICC, FC - P43488

Core Product Specifications and Parameters

Biological Background: CD252 (OX40 Ligand) Function and Localization

• CD252, also known as OX40 ligand (OX40L) and TNF superfamily member 4, is a type II membrane glycoprotein encoded by the Tnfsf4 gene in mice.
• It functions as a cytokine that binds to its receptor TNFRSF4 (OX40) to co-stimulate T-cell proliferation and cytokine production.
• CD252 is localized to the cell membrane, consistent with its role in cell-cell signaling during immune responses.
• The protein contains a signal-anchor, a transmembrane helix, and disulfide bonds, and is post-translationally modified by glycosylation.
• The mature soluble form may also be released, although membrane-bound expression is predominant for its co-stimulatory activity.

Experimental Guidance and Technical Tips

• The immunogen spans the extracellular domain (amino acids 51–198), which is likely accessible for cell surface staining in IF/ICC and FC applications.
• For flow cytometry (FC), consider titrating the antibody to determine the optimal staining concentration for your experimental system.
• For immunofluorescence (IF/ICC), validate fixation and permeabilization conditions, as the target is a membrane protein.
• Include appropriate isotype controls and confirm specificity in your model system before quantitative analysis.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories by providing access to validated antibodies like this CD252 (OX40 Ligand) monoclonal antibody for T-cell activation studies. As a comprehensive IVD raw material partner, we offer one-stop sourcing of monoclonal/polyclonal antibodies, bulk ancillary reagents, and technical consultation to accelerate your assay development from concept to clinic.