Rabbit anti-Mouse CD25 Monoclonal Antibody (IL2RA, p55) for Flow Cytometry - P01590

Core Product Specifications and Parameters

Biological Background: Interleukin-2 Receptor Subunit Alpha Function and Localization

• The target, interleukin-2 receptor subunit alpha (IL2RA), is also known as p55 or CD25 and is a type I transmembrane glycoprotein.
• It functions as a receptor for interleukin-2 (IL-2) and is a key component of the high-affinity IL-2 receptor complex.
• IL2RA plays a critical role in the regulation of immune tolerance by controlling the activity of regulatory T cells (TREGs). TREGs expressing CD25 suppress the activation and expansion of autoreactive T cells.
• The protein contains extracellular Sushi (CCP/SCR) domains involved in ligand binding and is anchored to the plasma membrane by a single transmembrane helix.
• It is a member of the type I cytokine receptor family and contains specific structural features such as a signal peptide, disulfide bonds, and N-linked glycosylation sites.
• Subcellular localization is predominantly at the cell membrane, consistent with its function as a surface cytokine receptor.

Experimental Guidance and Technical Tips

• The immunogen spans the extracellular domain (amino acids 22-236), making this antibody suitable for detecting the native surface receptor in non-permeabilized cells.
• Optimal working concentration should be determined by titration in the experimental context; a suggested starting range is 0.5–5 µg per 10^6 cells.
• Always include proper isotype controls and dead cell exclusion dyes when performing flow cytometry to ensure specific staining.
• Conjugated to the fluorophore, which has excitation and emission profiles similar to APC-Alexa Fluor 700, compatible with common flow cytometers.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials. This high-specificity CD25 antibody supports immune monitoring and tolerance research, and we offer validated antibody pairs, bulk ancillary reagents, and custom raw-material sourcing tailored to your assay development needs. Our portfolio covers key immunological targets, ensuring seamless procurement from concept to clinic.