Rabbit anti-Mouse CD25 Monoclonal Antibody for Flow Cytometry - P01590

Core Product Specifications and Parameters

Biological Background: CD25 Function and Localization

• CD25 is the alpha subunit of the interleukin-2 receptor (IL-2R), also known as p55, and is encoded by the Il2ra gene in mouse.
• It plays a central role in the regulation of immune tolerance by controlling regulatory T cell (Treg) activity, where Tregs suppress activation and expansion of autoreactive T cells.
• As a receptor for IL-2, CD25 participates in the high-affinity IL-2 receptor complex together with the beta and gamma chains, mediating signaling pathways critical for lymphocyte proliferation and homeostasis.
• CD25 is a type I transmembrane protein localized primarily on the plasma membrane, consistent with its function as a cell-surface receptor.
• The extracellular region contains two Sushi (CCP/SCR) domains and is modified by N-linked glycosylation and disulfide bonds, which contribute to structural stability and ligand interaction.

Experimental Guidance and Technical Tips

• The immunogen comprises the extracellular domain (amino acids 22-236) of mouse CD25, suggesting the antibody is likely to recognize native surface-expressed CD25.
• Designed for flow cytometry (FC), this ABflo 450 conjugate enables direct detection of CD25 without secondary staining; appropriate isotype controls and viability dyes should be considered to ensure specific staining.
• Titrate the antibody to determine the optimal concentration for your cell type and experimental conditions, and include relevant positive controls (e.g., activated T cells) and negative controls.
• In multicolor panels, verify minimal spillover and compensation requirements, particularly when combining ABflo 450 with other fluorophores in the violet laser channel.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines your journey from discovery to diagnostic application with a comprehensive portfolio of IVD raw materials. As a dedicated sourcing partner, we provide validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and tailored sourcing for rare targets. Whether you are developing Treg-focused immune tolerance assays or wider immunology panels, our supply chain and technical support accelerate your project’s progress from concept to clinic.