Rabbit anti-Mouse CD25 Monoclonal Antibody for Flow Cytometry - P01590

Core Product Specifications and Parameters

Biological Background: CD25 (IL-2Rα) Function and Localization

• CD25, also known as interleukin-2 receptor subunit alpha (IL-2Rα) or p55, is encoded by the Il2ra gene.
• It is a component of the high-affinity IL-2 receptor, which also includes IL-2Rβ and the common gamma chain (γc).
• CD25 is constitutively expressed on regulatory T cells (Tregs) and is essential for their suppressive function in immune tolerance.
• By binding IL-2, the receptor controls Treg activity, suppressing the activation and expansion of autoreactive T cells.
• Subcellular localization: single-pass type I membrane protein.
• The protein contains extracellular sushi (CCP) domains, a transmembrane helix, and is post-translationally modified by disulfide bonds and glycosylation.

Experimental Guidance and Technical Tips

• This antibody is directly conjugated to , a bright green fluorophore (Ex/Em: 490/515 nm), making it suitable for direct flow cytometry detection of mouse CD25 without a secondary antibody.
• For flow cytometry, titrate the antibody to determine the optimal staining concentration for your specific cell population.
• Consider using this antibody in combination with anti-CD4 and anti-FoxP3 antibodies for Treg cell identification (CD4+CD25+FoxP3+).
• Validate the antibody performance in your sample system, including appropriate isotype controls and Fc receptor blocking if necessary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers, laboratories, and research institutes with a comprehensive range of IVD raw materials, technical services, and consulting support. As a one-stop sourcing partner, we supply validated antibody pairs, optimized monoclonal antibodies like this anti-CD25 clone, and ancillary reagents to streamline your assay development from concept to clinic. Contact us for bulk procurement and custom sourcing solutions tailored to your cellular immunology and flow cytometry workflow needs.