Rabbit anti-Mouse CD25 Monoclonal Antibody for Flow Cytometry - P01590

Core Product Specifications and Parameters

Biological Background: Interleukin-2 receptor subunit alpha Function and Localization

• Also known as CD25 or p55, the interleukin-2 receptor subunit alpha (IL2RA) is a membrane protein that serves as the high-affinity receptor for interleukin-2 (IL-2).
• IL2RA plays a critical role in immune regulation by controlling regulatory T cell (Treg) activity. Tregs suppress the activation and expansion of autoreactive T cells, maintaining peripheral tolerance.
• Structurally, IL2RA contains characteristic sushi (CCP/SCR) domains, a single transmembrane helix, and is post-translationally modified with disulfide bonds and glycosylation.
• Encoded by the Il2ra gene (GeneID 16184 in mouse) with human ortholog GeneID 3559, this receptor subunit is essential for IL-2 signaling in T cells, B cells, and NK cells.
• Subcellularly localized to the plasma membrane, IL2RA is expressed on activated T lymphocytes and constitutively on Tregs.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 22-236), suggesting the antibody recognizes the native surface receptor; suitable for flow cytometry of live cells.
• Consider titrating the antibody on activated mouse splenocytes or Treg-enriched populations to determine optimal staining conditions.
• Pair with fluorophore-conjugated secondary antibodies for indirect detection, or directly conjugate for multicolor flow cytometry panels.
• Validate specificity in knockout or knockdown models if cross-reactivity with other species or isoforms is a concern, as only mouse reactivity is guaranteed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers with comprehensive IVD raw material sourcing, offering validated antibodies like CD25 clones for immune profiling. Whether you need bulk monoclonal antibodies, matched antibody pairs, or ancillary reagents for Treg and IL-2 pathway investigations, CamelBio streamlines procurement from concept to clinic. Our capabilities extend to rare-target antibody development and scalable production to accelerate your diagnostic project.