Rabbit anti-Mouse CD25 Monoclonal Antibody for FC - P01590

Core Product Specifications and Parameters

Biological Background: CD25 Function and Localization

• CD25, also known as interleukin-2 receptor subunit alpha (IL2RA) or p55, is encoded by the Il2ra gene and functions as the alpha chain of the IL-2 receptor.
• The receptor plays a critical role in immune tolerance by controlling the activity of regulatory T cells (TREGs), which suppress the activation and expansion of autoreactive T cells.
• CD25 is a type I transmembrane glycoprotein located on the cell membrane, containing sushi (complement control protein) domains and a transmembrane helix.
• The protein features structural elements such as a signal peptide, disulfide bonds, and a single transmembrane region, consistent with its classification as a membrane receptor.
• As indicated by its UniProt keywords, CD25 is involved in immunity and receptor-mediated signaling pathways.
• The immunogen used for this antibody covers the extracellular domain (amino acids 22–236), which includes the predicted ligand-binding region.

Experimental Guidance and Technical Tips

• This antibody is conjugated with , making it directly suitable for flow cytometry without the need for a secondary antibody.
• Since the immunogen targets the extracellular domain, the antibody is likely to recognize native surface CD25 on live mouse cells; consider optimizing staining protocols for your specific cell type.
• For mouse regulatory T cell identification, CD25 is commonly used in combination with other markers such as CD4 and FoxP3; validate the antibody in the relevant sample system.
• As with any reagent, individual titration is recommended to determine the optimal dilution for your experimental conditions.
• Avoid repeated freeze-thaw cycles to preserve the conjugate's stability and fluorescence intensity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with comprehensive access to IVD raw materials. For CD25-related immunological studies, we offer a range of validated antibody pairs and conjugated monoclonal antibodies. Our one-stop sourcing includes bulk ancillary reagents and flexible supply solutions to support your assay development from concept to clinic.