Rabbit anti-Mouse CD24 Monoclonal Antibody for IF/ICC and FC - P24807

Core Product Specifications and Parameters

Biological Background: CD24 Function and Localization

• CD24, also known as heat stable antigen (HSA), lymphocyte antigen 52 (Ly-52), and nectadrin, is a small, heavily glycosylated GPI-anchored cell surface protein encoded by the Cd24 gene (Gene ID: 12484).
• It plays a pivotal role in cell differentiation across various cell types, with a specific function in early thymocyte development (by similarity).
• Signaling is triggered by lectin-like ligand binding to CD24 carbohydrates, leading to release of second messengers from its GPI-anchor (by similarity).
• CD24 modulates B-cell activation responses (by similarity).
• In association with SIGLEC10, CD24 is involved in selective suppression of the immune response to danger-associated molecular patterns (DAMPs) such as HMGB1, HSP70, and HSP90 (PubMed:19264983). Related references: PMID:19264983
• CD24 plays a role in the control of autoimmunity (PubMed:20200274). Related references: PMID:20200274
• Subcellular localization: CD24 is anchored to the cell membrane via a GPI-linkage.
• Expressed in lymphoid, myeloid, and erythroid cells.

Experimental Guidance and Technical Tips

• Based on the immunogen (amino acids 27-53), the antibody likely recognizes an epitope in the N-terminal extracellular domain of mouse CD24. Consider this when designing epitope-mapping or competitive binding assays.
• This conjugate is directly fluorescent, simplifying IF/ICC and flow cytometry without secondary reagents. For IF/ICC, optimize fixation and permeabilization to preserve epitope integrity; methanol or paraformaldehyde fixation can be tested.
• For flow cytometry, perform titration to determine the optimal concentration for your cell type; use appropriate isotype controls. Note that CD24 is expressed on lymphoid, myeloid, and erythroid cells, so gating strategies may need to account for broad expression.
• While not listed for Western blotting, the predicted molecular weight is ~8 kDa (core protein), but the mature glycosylated form may appear larger. If WB is attempted, use high-percentage gels and consider glycosidase treatment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with integrated access to high-quality IVD raw materials, including validated antibody pairs, optimized monoclonal antibodies like this anti-CD24 clone, and bulk ancillary reagents. Our expertise in rare target sourcing and technical support helps streamline your development of immunoassays for immune cell profiling, autoimmune disease research, and beyond. Whether you need custom conjugations or scale-up production, CamelBio bridges the gap from concept to clinic.