Rabbit anti-Mouse CD24 Monoclonal Antibody for Flow Cytometry - P24807

Core Product Specifications and Parameters

Biological Background: Signal transducer CD24 Function and Localization

• CD24, also known as HSA, Ly-52, or nectadrin, is a heavily glycosylated GPI-anchored cell surface protein expressed in lymphoid, myeloid, and erythroid cells.
• It plays a pivotal role in cell differentiation across various cell types and is particularly important in early thymocyte development.
• Signaling through CD24 is thought to be triggered by lectin-like ligand binding to its carbohydrates and transduced via second messengers released from the GPI anchor.
• CD24 modulates B-cell activation responses (By similarity).
• In association with SIGLEC10, CD24 participates in the selective suppression of immune responses to danger-associated molecular patterns (DAMPs) such as HMGB1, HSP70, and HSP90. Related references: PMID:19264983
• The protein also plays a role in the control of autoimmunity. Related references: PMID:20200274
• CD24 is localized exclusively at the cell membrane.
• UniProt keywords include: Cell membrane, Direct protein sequencing, Glycoprotein, GPI-anchor, Immunity, Innate immunity, Lipoprotein, Membrane, Reference proteome, Signal.

Experimental Guidance and Technical Tips

• This antibody is directly conjugated to , a violet-excitable fluorochrome. It is recommended for flow cytometry applications using appropriate filter sets (e.g., 405 nm excitation with 450/50 nm emission).
• Titrate the antibody to determine the optimal staining concentration for your specific experimental system.
• Include isotype control antibodies (e.g., rabbit IgG conjugated to ) to assess background staining.
• Protect from light during staining and storage to maintain fluorochrome stability.
• Proper compensation controls (e.g., single-stained cells or beads) are essential when combining with other fluorochromes.
• Validate staining protocols in your cell type of interest, especially if working with rare populations or low antigen expression.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research institutes with reliable IVD raw materials, including validated monoclonal antibodies like this anti-CD24 clone, bulk ancillary reagents, and rare-target sourcing. Our integrated supply capabilities support your CD24-related projects from early-phase research to clinical assay development, ensuring consistent quality and technical support at every stage.