Rabbit anti-Mouse CD23 Monoclonal Antibody for Flow Cytometry - P20693

Core Product Specifications and Parameters

Biological Background: CD23 Function and Localization

• CD23, also known as Fc-epsilon-RII or Lymphocyte IgE receptor, is encoded by the Fcer2 gene (GeneID: 14128). It is a low-affinity receptor for immunoglobulin E (IgE) and complement receptor 2 (CD21).
• CD23 plays a critical role in regulating IgE production and B cell differentiation. On B cells, it facilitates IgE-dependent antigen uptake and presentation to T cells, linking humoral and cellular immunity.
• On macrophages, CD23 engagement by IgE-antigen complexes triggers intracellular parasite killing via the L-arginine-nitric oxide pathway.
• Alternative splicing generates both membrane-bound and secreted isoforms of CD23, with the secreted form potentially modulating IgE-mediated responses extracellularly.
• The protein localizes primarily on the cell membrane, anchored by a signal-anchor transmembrane helix; a secreted form is also produced.
• Post-translational modifications include glycosylation, palmitoylation, and disulfide bond formation, which may influence its stability and function. It is classified as a calcium-dependent lectin and proteoglycan.
• CD23 is widely used as a B cell marker in mouse immunology, particularly for studying IgE-associated allergies and asthma.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular region (amino acids 50-331) of mouse CD23; therefore, this antibody is expected to detect surface-expressed CD23 on live cells.
• For flow cytometry, titrate the antibody to find the optimal concentration for your specific cell type and experimental conditions. Use an appropriate isotype control to assess non-specific binding.
• Consider that Fcer2 expression can be upregulated on B cells and macrophages upon activation; validate detection in your model system under relevant stimuli.
• Avoid freeze-thaw cycles and protect the conjugate from prolonged light exposure to maintain fluorophore stability.
• If combining with other fluorescent antibodies, ensure minimal spectral overlap with (excitation 488 nm, emission ~520 nm).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic and research labs with one-stop access to IVD raw materials, including validated antibodies, recombinant proteins, and ancillary reagents. This anti-mouse CD23 antibody is part of our extensive portfolio supporting immunology and allergy research. In addition to off-the-shelf products, we offer customized sourcing for rare targets, bulk quantities, and technical consultation to streamline your assay development.