Rabbit anti-Mouse CD23 Monoclonal Antibody for Flow Cytometry - P20693

Core Product Specifications and Parameters

Biological Background: CD23 Function and Localization

• CD23, also known as Fc-epsilon-RII or low affinity immunoglobulin epsilon Fc receptor, is a type II transmembrane protein that acts as a receptor for IgE and CR2/CD21. It exists in two isoforms (CD23a and CD23b) generated by alternative splicing, differing in their cytoplasmic domains.
• On B cells, CD23 is a key regulator of IgE synthesis and B cell differentiation. It mediates IgE-dependent antigen capture and subsequent presentation to T cells, thereby influencing humoral immunity.
• In macrophages, IgE binding and antigen cross-linking via CD23 triggers intracellular killing of parasites through activation of the L-arginine-nitric oxide pathway.
• CD23 localizes primarily at the cell membrane but can also be released as a soluble receptor by proteolytic cleavage, acting as a secreted molecule that further modulates immune responses.
• The protein undergoes post-translational modifications including N-linked glycosylation, palmitoylation, and disulfide bond formation. It also contains a C-type lectin domain and a calcium-binding site.
• As indicated by UniProt keywords, CD23 is a lipoprotein, proteoglycan, and IgE-binding protein, highlighting its diverse biochemical roles and membrane association.

Experimental Guidance and Technical Tips

• The immunogen used for this antibody was a recombinant fusion protein covering amino acids 50-331 of mouse CD23. This region encompasses the extracellular lectin domain, ensuring specific recognition of the native protein.
• The antibody is directly conjugated to , a far-red fluorophore compatible with common flow cytometry instruments. Its excitation/emission profile (∼686/713 nm) allows for good separation from other common dyes like FITC, PE, or APC.
• For flow cytometry, consider testing the antibody at a range of dilutions (e.g., 0.5–5 µg/mL) on mouse splenocytes or peripheral blood leukocytes, where CD23 is expressed on B cells and some myeloid cells.
• Include appropriate isotype controls and Fc receptor blocking steps to minimize non-specific binding, especially in samples with high FcγR expression.
• Since the predicted molecular weight is ~37 kDa (unglycosylated) but the mature glycoprotein migrates at ~45–50 kDa on SDS-PAGE, this antibody may not be suitable for western blotting unless reduced/denatured conditions are carefully optimized. Its reported application is specifically flow cytometry.
• Validate the staining in your specific mouse strain and cell subset of interest, as CD23 expression levels can vary with activation status and microenvironment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers by providing reliable access to specialized IVD raw materials like this anti‑mouse CD23 monoclonal antibody, ideal for immunological studies and B‑cell profiling. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, and ancillary reagents such as conjugated secondary antibodies, enabling seamless integration into flow cytometry‑based assays. From bulk procurement of core antibodies to sourcing rare‑target reagents, CamelBio streamlines your supply chain with a single partner dedicated to rapid, dependable delivery.